Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources : 세 계통의 ICR 마우스에서의 2,2,2-tribromoethanol 마취효과 비교연구

국문 초록 (Abstract)

본 연구는 세 계통의 ICR 마우스에서의 2,2,2-tribromoethanol 마취효과에 대한 비교 연구이다. 고용량 (500mg/kg), 중간용량 (250mg/kg), 저용량 (125mg/kg)의 2.5% TBE를 복강 내 투여하였으며, 마취시간, 회복시간, 호기말 이산화탄소 농도, 평균동맥혈압, 심박수, 산소포화도, 체온, 산도, 동맥혈 이산화탄소 및 산소분압을 측정하였다. 모든 농도에서 마취는 안정적으로 유도되었다. 마취시간은 용량의존적으로 나타났으며 세 계통에서 뚜렷한 차이를 보이지 않았다. 마취시간은 수컷보다 암컷에서 길게 나타났으며, 고용량 그룹에서 수컷보다 암컷에서 뚜렷하게 길게 측정되었다. 회복시간은 중간용량과 고용량 그룹에서 수컷보다 암컷에서 뚜렷하게 길게 나타났다. 평균동맥혈압, 산소포화도, 동맥혈 이산화탄소 및 산소분압은 마취 후 감소하였고 심박수와 호기말 이산화탄소 농도는 마취 후 증가하였으며 세 계통간 차이는 없었다. 마취에 의한 체온, 혈액 내 생화학적 지표, 조직병리학적 변화는 나타나지 않았다. 세 계통에서의 ICR 마우스에 TBE 단회투여시 유사한 반응을 보였다. 실험결과를 바탕으로 TBE는 세 계통의 ICR 마우스에서 비슷한 마취효과를 유도하는 효과적인 약물로 사용될 수 있을것으로 판단된다.

다국어 초록 (Multilingual Abstract)

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCO2), mean arterial blood pressure, heart rate, oxygen saturation (SpO2), body temperature, pH, PCO2, and PO2 of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different sources. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, the mean arterial blood pressure, SPO2, arterial blood PCO2, and PO2, were decreased after TBE anesthesia, and heart rate and ETCO2 were increased after TBE anesthesia. But there were no significant differences in the three different sources. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in ICR mice from three different sources.

목차 (Table of Contents)

• CONTENTS
• ABSTRACT...................................................................................................1
• INTRODUCTION........................................................................................3
• MATERIALS AND METHOD...................................................................5
• Animal experiment........................................................................5
• Preparation of anesthetic agent.....................................................6
• Anesthetic monitoring and physiological parameters...................7
• Anesthesia time and recovery time monitoring….........................8
• Blood pressure, heart rate, body temperature, end-tidal peak CO2 and SpO2 measurements......................8
• Analysis of arterial blood gases and serum biochemistry.............9
• Histopathological examination....................................................10
• Statistical analysis.......................................................................10
•  
• RESULTS....................................................................................................11
• Anesthesia time and anesthesia recovery time……....................11
• Mean arterial blood pressure, heart rate, end-tidal peak CO2, and SpO2 ……………………………13
• Arterial blood PCO2, PO2, and pH …………………………14
• Blood biochemistry.....................................................................15
• Histopathological changes in the liver, kidney, and lung............15
• FIGURES AND LEGENDS.......................................................................17
• TABLES.......................................................................................................23
• DISCUSSION .............................................................................................26
• REFERENCES...........................................................................................34
• ABSTRACTS IN KOREAN..................................................................... 41