The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 β-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for β-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the...
The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 β-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for β-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the nucleotide sequence of β-xylosidase gene, agreed with the result obtained by SDS-polyacrylamide gel electrophoresis of the purified enzyme (51,000 dalton). The Shine-Dalgarno sequence, 5'-GAGGAGG-3', was found 8 bp upstream of the initiation codon ATG. The -10 sequence (TAAAAT) in the promoter region for β-xylosidase gene was similar to the consensus sequence for Bacillus subtilis RNA polymerase, whereas the -35 sequence (TCGATCA) different from all the known -35 regions in the promoter for Bacillus subtilis RNA polymerase.