자일리톨 함유 식품이 합성 수산화인회석에 대한 Streptococcus mutans의 부착에 미치는 영향에 관한 실험적 연구 : An in vitro study = An effect of Xylitol on the Adhesiveness of Streptococcus mutans to Synthetic Hydroxyapatite

다국어 초록 (Multilingual Abstract)

There have been efforts that inhibit development of dental caries by sugar substitution. But, it is controversial if xylitol has anticariogenic effect in the presence of sucrose. And there are few papers dealing with the combined action of xylitol and sucrose. For the purpose of resolving this controversy, the author investigated the effect of xylitol on enamel demineralization and on adhesiveness of S. mutans to hydroxyapatite in the presence of sucrose.
Five experimental solutions were prepared as follows: (S: sucrose, X: xylitol)
Group 1: BHI broth Group 2: BHI + 1 % S
Group 3: BHI + 0.75% S+0.25% X Group 4: BHI + 0.5% S + 0.5% X
Group 5: BHI + 0.25% S + 0.75% X Group 6: BHI + 1 % X
Each solution was inoculated with 100㎕ of S. mutans JC-2. And saliva coated hydroxyapatite beads were put into each experimental solution. And then each solution was incubated at 37℃ under anaerobic condition. After incubation, the adhesiveness of S. mutans on hydroxyapatite was evaluated. The Vickers hardness numbers were measured on extracted human primary teeth, and these teeth were dipped into the same experimental solution and incubated at 37℃ under anaerobic condition for 48hours. Surface microhardness were measured again after incubation.
The obtained results were as follows;
1. In the presence of sucrose, xylitol can reduce the adhesiveness of S. mutans on hydroxyapatite surface from the ratio of 25% sucrose to 75% xylitol(P<0.05).
2. In the presence of sucrose, xylitol can reduced demineralization of primary teeth enamel surface from the ratio of 50% sucrose to 50% xylitol(P<0.01).

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 연구재료 및 방법
• Ⅲ. 연구성적
• Ⅳ. 총괄 및 고찰
• Ⅴ. 결론
• 참고문헌
• ABSTRACT