The first record of nine bacterial species belonging to the phylum Proteobacteria in Korea

다국어 초록 (Multilingual Abstract)

As part of a larger study with the aim to discover indigenous prokaryotic species in Korea, nine bacterial strains were isolated and assigned to the phylum Proteobacteria in 2016. High 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clades with known species indicated that each strain belongs to an independent and predefined bacterial species. This is the first report of these nine species in Korea: two strains of the Methylobacterium, two strains of the Microvirga, one strain of the Pantoea, and four strains of the Psychrobacter, all within the Proteobacteria. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

참고문헌 (Reference)

1 Kimura, M., "The neutral theory of molecular evolution" Cambridge University Press 1983

2 Saitou, N., "The neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

3 Yumoto, I., "Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int" 53 : 1985-1989, 2003

4 Romanenko, L. A., "Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan" 54 : 1741-1745, 2004

5 Bakermans, C., "Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost" 56 : 1285-1291, 2006

6 Williams, K. P., "Phylogeny of gammaproteobacteria" 192 : 2305-2314, 2010

7 Bowman, J. P., "Novel Psychrobacter species from Antarctic ornithogenic soils" 46 : 841-848, 1996

8 Kanso, S., "Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer" 53 : 401-406, 2003

9 Ardley, J. K., "Microvirga lupini sp. nov., Microvirga lotononidis sp. nov. and Microvirga zambiensis sp. nov. are alphaproteobacterial root-nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts" 62 : 2579-2588, 2012

10 Cao, Y. -R., "Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil" 99 : 629-634, 2011

11 Gallego, V., "Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water" 55 : 281-287, 2005

12 Edgar, R. C., "MUSCLE: multiple sequence alignment with high accuracy and high throughput" 32 : 1792-1797, 2004

13 Tamura, K., "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods" 28 : 2731-2739, 2011

14 Kim, O. -S., "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species" 62 : 716-721, 2012

15 Brady, C. L., "Emended description of the genus Pantoea, description of four species from human clinical samples, Pantoea septica sp. nov., Pantoea eucrina sp. nov., Pantoea brenneri sp. nov. and Pantoea conspicua sp. nov., and transfer of Pectobacterium cypripedii (Hori 1911) Brenner et al. 1973 emend. Hauben et al. 1998 to the genus as Pantoea cypripedii comb. nov" 60 : 2430-2440, 2010

16 Doetsch, R., "Determinative methods of light microscopy"

17 Felsenstein, J., "Confidence limits on phylogenies: an approach using the bootstrap" 39 : 783-791, 1985

18 Wragg, P., "Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest" 105 : 16-21, 2014

19 Hall, T.A., "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT" Information Retrieval Ltd. 41 : 95-98, 1999

20 Woese, C.R., "Bacterial evolution" 51 : 221-271, 1987

21 Williams, K. P., "A robust species tree for the alphaproteobacteria" 189 : 4578-4586, 2007

22 Weisburg, W. G., "16S ribosomal DNA amplification for phylogenetic study" 173 : 697-703, 1991