A Candidate Single Nucleotide Polymorphism in the 3′ Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs

다국어 초록 (Multilingual Abstract)

Fatness qualities in pigs measured by the amount of fat deposition and composition of fatty acids (FAs) in pork have considerable effect on current breeding goals. The stearoyl-CoA desaturase (SCD) gene plays a crucial role in the conversion of saturated FAs into monounsaturated FAs (MUFAs), and hence, is among the candidate genes responsible for pig fatness traits. Here, we identified a single nucleotide polymorphism (SNP, c.*2041T>C) in the 3′ untranslated region by direct sequencing focused on coding and regulatory regions of porcine SCD. According to the association analysis using a hundred of Berkshire pigs, the SNP was significantly associated with FA composition (MUFAs and polyunsaturated FAs [PUFAs]), polyunsaturated to saturated (P:S) FA ratio, n-6:n-3 FA ratio, and extent of fat deposition such as intramuscular fat and marbling (p<0.05). In addition, the SNP showed a significant effect on the SCD mRNA expression levels (p = 0.041). Based on our results, we suggest that the SCD c.*2041T>C SNP plays a role in the gene regulation and affects the fatness qualities in Berkshire pigs.

참고문헌 (Reference)

1 Shabalina, S. A., "The mammalian transcriptome and the function of non-coding DNA sequences" 5 (5): 105-, 2004

2 Bentley, G., "The health effects of dietary unsaturated fatty acids" 32 : 82-84, 2007

3 Dobrzyn, A., "Stearoyl-CoA desaturase - A new player in skeletal muscle metabolism regulation" 57 : 31-42, 2006

4 Jiang, Z., "Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle" 25 : 345-351, 2008

5 Gerin, I., "Roles for miRNA-378/378* in adipocyte gene expression and lipogenesis" 299 : 198-206, 2010

6 Ntambi, J. M., "Regulation of stearoyl-CoA desaturase expression" 39 : 1061-1065, 2004

7 Ntambi, J. M., "Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol" 40 : 1549-1558, 1999

8 Miyazaki, M., "Oleoyl-CoA is the major de novo product of stearoyl-coa desaturase 1 gene isoform and substrate for the biosynthesis of the harderian gland 1-alkyl-2,3-diacylglycerol" 276 : 39455-39461, 2001

9 AOAC., "Official Methods of Analysis" Association of Official Analytical Chemists 2000

10 Enoch, H. G., "Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid" 251 : 5095-5103, 1976

11 Suzuki, K., "Meat quality comparison of Berkshire, Duroc and crossbred pigs sired by Berkshire and Duroc" 64 : 35-42, 2003

12 Smet, S. D., "Meat fatty acid composition as affected by fatness and genetic factors: a review" 53 : 81-98, 2004

13 Wood, J. D., "Manipulating meat quality and composition" 58 : 363-370, 1999

14 Ren, J., "Isolation and molecular characterization of the porcine stearoyl-CoA desaturase gene" 340 : 19-30, 2004

15 Uemoto, Y., "Genome‐wide mapping for fatty acid composition and melting point of fat in a purebred Duroc pig population" 43 : 27-34, 2012

16 Chen, P., "Genetic parameters and trends for lean growth rate and its components in U.S. Yorkshire, Duroc, Hampshire, and Landrace pigs" 80 : 2062-2070, 2002

17 Cameron, N. D., "Genetic and phenotypic parameters for carcass traits, meat and eating quality traits in pigs" 26 : 119-135, 1990

18 NPPC., "Genetic Evaluation, Terminal Line Program Results" National Pork Producers Council 1995

19 Voss, M. D., "Gene expression profiling in skeletal muscle of Zucker diabetic fatty rats : implications for a role of stearoyl-CoA desaturase 1 in insulin resistance" 48 : 2622-2630, 2005

20 Uemoto, Y., "Fine mapping of porcine SSC14 QTL and SCD gene effects on fatty acid composition and melting point of fat in a Duroc purebred population" 43 : 225-228, 2012

21 Hulver, M. W., "Elevated stearoyl-CoA desaturase-1 expression in skeletal muscle contributes to abnormal fatty acid partitioning in obese humans" 2 : 251-261, 2005

22 Kim, J. M., "Effects of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 polymorphisms on fat androstenone level and gene expression in Duroc pigs" 44 : 592-595, 2013

23 Wood, J. D., "Effects of fatty acids on meat quality: A review" 66 : 21-32, 2003

24 Wood, J. D., "Effects of breed, diet and muscle on fat deposition and eating quality in pigs" 67 : 651-667, 2004

25 Erkkilä, A., "Dietary fatty acids and cardiovascular disease: An epidemiological approach" 47 : 172-187, 2008

26 Ren, J., "Characterization of five single nucleotide polymorphisms in the porcine stearoyl-CoA desaturase (SCD) gene" 35 : 255-257, 2004

27 Maharani, D., "Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs" 40 : 73-80, 2013

28 Lee, S. H., "Association between polymorphisms of the heart fatty acid binding protein gene and intramuscular fat content, fatty acid composition, and meat quality in Berkshire breed" 86 : 794-800, 2010

29 Ren, J., "Assignment of the porcine stearoyl-CoA desaturase (SCD) gene to SSC14q27 by fluorescence in situ hybridization and by hybrid panel mapping" 34 : 471-473, 2003

30 Livak, K. J., "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method" 25 : 402-408, 2001

31 Folch, J., "A simple method for the isolation and purification of total lipides from animal tissues" 226 : 497-509, 1957

32 Estany, J., "A functional variant in the stearoyl-coa desaturase gene promoter enhances fatty acid desaturation in pork" 9 (9): e86177-, 2014