Evaluating sulfoxaflor residues in pig tissues using animal modeling

다국어 초록 (Multilingual Abstract)

Maximum residue limits (MRL) for pesticides in feed have been set to protect public healthand produce safe livestock products. In vivo experiments to establish MRL are essential,as livestock are commonly used to obtain reliable in vivo quantitative information. Here, weaimed to evaluate whether small laboratory animals can replace or reduce monogastric livestockin experiments to quantify pesticide residues in vivo after oral consumption throughfeed. First, 24 pigs and rats were randomly assigned to four groups and fed 0, 3, 9, or 30 mg/kg of sulfoxaflor. After four weeks, serum, muscle, fat, liver, kidney, and small intestine sampleswere collected, and sulfoxaflor residues were analyzed using liquid chromatography –tandem mass spectrometry. Sulfoxaflor residues in pig tissues were significantly correlatedwith those in rat tissues. Model equations were formulated based on the residual sulfoxafloramount in pig and rat tissues. The calculated and measured sulfoxaflor residues in pigs andrats showed more than 90% similarity. Sulfoxaflor did not affect body weight gain, feed intake,or the feed conversion ratio. Therefore, we concluded that pesticide residue quantification invivo to establish MRL could be performed using small laboratory animals instead of livestockanimals. This would contribute to obtaining in vivo pesticide residue information and reducinglarge-scale livestock animal experiments.

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