<i>Oryzihumus terrae</i> sp. nov., isolated from soil and emended description of the genus <i>Oryzihumus</i>

다국어 초록 (Multilingual Abstract)

<P>A Gram-stain-positive, aerobic, non-flagellated bacterium, designated KIS22-12<SUP>T</SUP>, was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. Cells were non-spore-forming cocci showing catalase-positive and oxidase-negative reactions. Growth of strain KIS22-12<SUP>T</SUP> was observed between 10 and 35 °C (optimum, 28–30 °C), between pH 5.0 and 9.0 (optimum, pH 7.0) and with 0–3 % (w/v) NaCl. Strain KIS22-12<SUP>T</SUP> contained MK-8(H<SUB>4</SUB>) as the predominant menaquinone, and C<SUB>17 : 1</SUB>ω8<I>c</I>, iso-C<SUB>15 : 0</SUB> and anteiso-C<SUB>15 : 0</SUB> as the major fatty acids. Strain KIS22-12<SUP>T</SUP> contained diphosphatidylglycerol, phosphatidylinositol, one unknown aminophospholipid, one unknown aminolipid, two unknown phospholipids and one unknown lipid. The peptidoglycan type was A1γ. The G+C content of the genomic DNA was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KIS22-12<SUP>T</SUP> formed a phyletic lineage with <I>Oryzihumus leptocrescens</I> KV-628<SUP>T</SUP>. 16S rRNA gene sequence similarity between the two strains was 96.5 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain KIS22-12<SUP>T</SUP> represents a novel species within the genus <I>Oryzihumus</I>, for which the name <I>Oryzihumus terrae</I> sp. nov. is proposed. The type strain is KIS22-12<SUP>T</SUP> ( = KACC 16543<SUP>T</SUP> = DSM 27161<SUP>T</SUP> = NBRC 109596<SUP>T</SUP>). An emended description of the genus <I>Oryzihumus</I> is also provided.</P>