Tunicamycin 이 돼지정자의 생존성, 미토콘드리아 활성, 정자의 운동성에 미치는 영향

다국어 초록 (Multilingual Abstract)

This study was to investigate effect of tunicamycin (TM) on sperm viability, mitochondrial activity and motility in boar semen. Collected sperm were incubated with semen extender containing 0, 1, 2, and 5 μM TM for 3, 6 and 9 h. Sperm viability was analyzed using SYBR14/PI doubling staining, and mitochondrial activity was detected using Rhodamine123/PI staining methods. Sperm viability, mitochondrial activity and motility were measured every 3 h during incubation. In results, boar sperm viability, mitochondrial activity and motility were significantly decreased in 2 and 5 μM TM groups compare to control group at all incubation time (p<0.05). In addition, mitochondrial activity and motility were significantly decreased in 1, 2, and 5 μM TM groups compare to control group at 9 h after incubation (p<0.05). These results suggest that TM can inhibit sperm viability, mitochondrial activity and motility in boar semen, and it may influence on the fertility of sperm.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• 1. 정액 준비
• 2. 정자의 생존성
• 3. 미토콘드리아 활성 검사
• 4. 정자 운동성
• 5. 통계처리
• Ⅲ. 결과
• 1. Tunicamycin(TM)이 정자의 생존성의 미치는 영향
• 2. 정자의 미토콘드리아 활성에 미치는 tunicamycin(TM)의 영향
• 3. Tunicamycin(TM)이 정자 운동성에 미치는 영향
• Ⅳ. 고찰
• Ⅴ. 요약
• Ⅵ. 참고문헌

참고문헌 (Reference)

1 이상희, "미니돼지 정액 보존에 있어서 해양성 광천수의 영향" 동물자원공동연구소 21 (21): 19-25, 2010

2 Hou, H., "Tunicamycin potentiates cisplatin anticancer efficacy through the DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma" 12 : 2874-2884, 2013

3 Schwarz, D. S., "The endoplasmic reticulum: structure, function and response to cellular signaling" 73 : 79-94, 2016

4 Baumber, J., "The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation" 21 : 895-902, 2000

5 Kim, J. S., "Tauroursodeoxycholic acid enhances the preimplantation embryo development by reducing apoptosis in pigs" 47 : 791-798, 2012

6 Bungum, M., "Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome" 22 : 174-179, 2006

7 Salicioni, A. M., "Signalling pathways involved in sperm capacitation" 245-249, 2007

8 Ax, R., "Semen evaluation. In Reproduction in Farm Animals" Blackwell Publishing 363-375, 2000

9 Lay, K., "N-glycosylation of zona glycoproteins during meiotic maturation is involved in sperm-zona pellucida interactions of porcine oocytes" 75 : 1146-1152, 2011

10 Grootegoed, J. A., "Molecular and cellular mechanisms in spermatogenesis" 14 : 331-343, 2000

11 Hess, R. A., "Molecular Mechanisms in Spermatogenesis" Springer 1-15, 2009

12 Stewart, P., "Lack of effect of tunicamycin on spermatogenesis in rams" 76 : 289-290, 1998

13 Tabas, I., "Integrating the mechanisms of apoptosis induced by endoplasmic reticulum stress" 13 : 184-190, 2011

14 Zhang, J. Y., "Inhibition of endoplasmic reticulum stress improves mouse embryo development" 7 : e40433-, 2012

15 Steinberger, E., "Hormonal control of mammalian spermatogenesis" 51 : 1-22, 1971

16 Zhang, J. Y., "Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1) in the porcine system" 12 : 1-11, 2011

17 Abraham, T., "Embryo collection induces transient activation of XBP1 arm of the ER stress response while embryo vitrification does not" 18 : 229-242, 2011

18 이아성, "Effects of Streptozotocin, Bisphenol A and Diethylstilbestrol on Production of Reactive Oxygen Species and Lipid Peroxidation in the Boar Sperm" 대한의생명과학회 23 (23): 128-132, 2017

19 Lee, S. H., "Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen" 154 : 86-94, 2015

20 Zhang, J. Y., "Effect of endoplasmic reticulum stress on porcine oocyte maturation and parthenogenetic embryonic development in vitro" 86 : 1-9, 2012

21 Sakaguchi, Y., "Changes in the sperm-zona pellucida binding properties during porcine oocyte maturation" 27 : 130-135, 2010

22 Carreau, S., "Aromatase, oestrogens and human male reproduction" 365 : 1571-1579, 2010

23 Nakamura, M., "An endoplasmic reticulum protein, calreticulin, is transported into the acrosome of rat sperm" 205 : 101-110, 1993

24 Chaudhari, N., "A molecular web: endoplasmic reticulum stress, inflammation, and oxidative stress" 8 : 1-15, 2014