저농도 산분해방법에 의한 기능성 키틴, 키토산 가수분해물의 제조 및 이용 = Production and Utilization of the Functional Hydrolysates of Chitin and Chitosan under Low Acid conditions

다국어 초록 (Multilingual Abstract)

Chitin and chitosan were hydrolyzed by microwaving and autoclaving treatment in a low concentration of hydrochloric and organic acid in order to develop new hydrolysis methods under low pollution conditions.
For high yield of chitin hydrolysates prepared by physicochemical method in a low concentration of HCI, a microwaving treatment was more effective than autoclaving treatment. The molecular weight of chitin was lower, the yield of chitin hydrolysates was higher.
Through a physicochemical method using a 0.575 organic acid, a higher yield of chitosan hydrolysates was reached through a microwaving treatment than autoclaving treatment. The molecular weight of chitosan was lower, the yield of chitosan hydrolysates was better. Among the organic acids, 0.5% succinic acid was the best solution in physicochemical hydrolysis of chitosan.
Chitosan hydrosates were higher antimicrobial activity than chitin hydrolysates. In minimum inhibitory concentration on the growth of bacteria, chitosan hydrolysate was lower than its oligosaccharides fraction(MW< 10,000). All the hydrolysates of chitin and chitosan had anticavitied activity, not the ACE inhibitory activity.
Chitosan hydrolysate prepared by microwaving treatment in 0.5% succinic acid for 200min at 121 ℃ was added into fish paste meat, then the change of quality of fish paste meat was measured during storage at 5℃. Until 18th day in storage, viable cell count was lower than the control, jelly strength and hardness was high.