絡石藤약침이 Collagen 유발 관절염에 미치는 영향 = Effect of Trachelospermi Caulis Herbal-acupuncture on the Collagen-induced Arthritis in Rats

다국어 초록 (Multilingual Abstract)

Objectives : This study was built to investigate the effect of Trachelospermi Caulis herbal- acupuncture on the Collagen-induced arthritis(CIA) in rats.
Methods : Arthritis was induced by intradermal injection of Bovine type Ⅱ collagen solution into base of tail. Experimental group were divided into 5 groups ; Normal(N) group, Control(C) group, Trachelospermi Caulis high(TH) group, Trachelospermi Caulis low(TL) group, Saline(S) group(n=7 for each group). Normal group was had no management. Control group was injected with Bovine type Ⅱ collagen solution and taken no treatment. Trachelospermi Caulis high group was injected with Bovine type Ⅱ collagen solution and taken high-intensity(10㎎/㎏) herbal-acupuncture treatment on ST36. Trachelospermi Caulis low group was injected with Bovine type Ⅱ collagen solution and taken low-intensity(5㎎/㎏) herbal-acupuncture treatment on ST36. Saline group was injected with Bovine type Ⅱ collagen solution and taken saline injection on ST36. Body weight, paw edema volume and ankle joint thickness were measured during experimental day. On the last experimental day, we analyzed WBC count, TNF-α & IL-1β concentration, c-fos immunohistochemistry and NADPH-d histochemistry for evaluating the effect of Trachelospermi Caulis herbal-acupuncture.
Results : The results were as follows ;
1. In the change of paw edema volume, TH group only has significant difference compared with C group.
2. In the change of ankle joint thickness, TH group only has significant difference compared with C group.
3. In WBC count of serum, TH, TL groups have significant decrease compared with C group.
4. In TNF-α concentration of effusion, TH, TL groups have significant decrease compared with C group. and TH group has significant decrease compared with TL group.
5. In IL-1β concentration of effusion, TH, TL groups have significant decrease compared with C group. and TH group has significant decrease compared with TL group.
6. In c-fos positive neurons of S1S2(cortex) region, TH, TL, S groups have significant decrease compared with C group.
7. In NADPH-d positive neurons of CPu(caudate putamen) region, TH, TL groups have significant decrease compared with C group.
8. In NADPH-d positive neurons of Tfp(transverse fibers of pons) region, TH, TL, S groups have significant decrease compared with C group. and TH group has significant decrease compared with S group.
Conclusions : According to above results, we hope that Trachelospermi Caulis herbal-acupuncture may have the effect that decreases progression and development of CIA. And it can be suggested that Trachelospermi Caulis herbal-acupuncture may reduce the expression of c-fos and NOS.

참고문헌 (Reference)

1 프리미어 의학연구회, "프리미어 내과 volume 4" 고려의학 2083-2090, 2008

2 신규성, "파워내과학 4" 군자출판사 286-297, 2004

3 대한정형외과학회, "정형외과학 6판" 최신의학사 232-247, 2006

4 서울대학교 의과대학 내과학교실, "임상내과학(Ⅱ)" 고려의학 2067-2079, 2004

5 이창환, "유근피 약침이 제2형 Collagen 관절염에서 MIF 활성 억제 및 T세포 분화 조절에 미치는 영향" 대한침구학회 25 (25): 83-96, 2008

6 전국의과대학교수, "오늘의 진단 및 치료" 한우리 892-907, 2000

7 권용화, "염증성 관절염에서 Nitric Oxide(NO)의 역할에 관한 연구" 16 (16): 227-236, 1996

8 박동권, "백서의 실험적 치아이동시 Nitric Oxide Synthetase의 발현 양상" 31 (31): 107-120, 2001

9 박정숙, "류마티스 관절염환자의 피로에 대한 조사" 7 (7): 245-253, 2001

10 이주희, "류마티스 관절염 환자의 항산화 영양소 섭취상태와 생활습관" 대한지역사회영양학회 13 (13): 253-262, 2008

11 장세영, "류마티스 관절염 환자의 원인지각, 대응양상, 사회심리적 적응과의 관계" 9 (9): 187-202, 2002

12 한정원, "류마티스 관절염 환자의 무기력과 건강행위에 관한 연구" 대한근관절건강학회 15 (15): 33-40, 2008

13 유경희, "류마티스 관절염 환자가 지각하는 통증과 우울 정도 및 관련 요인" 8 (8): 189-198, 2001

14 최경숙, "류마티스 관절염 환자 배우자의 부담감" 7 (7): 241-257, 2000

15 김성윤, "류마토이드 관절염의 면역병인" 33 (33): 577-580, 1987

16 서영배, 신미경, "동충하초 추출물이 콜라겐으로 유발된 관절염 생쥐 모델에 미치는 영향" 대한본초학회 22 (22): 57-65, 2007

17 이현진, "녹용약침이 백서의 제2형 Collagen 유발 관절염에서 iNOS 발현과 NO 생성 억제에 미치는 영향" 대한침구학회 25 (25): 105-116, 2008

18 김형섭, "내과학 5" 고려의학 279-294, 2003

19 미국정형외과학회, "근골격계 진단 및 치료의 핵심" 한우리 57-58, 2001

20 김은정, "電鍼의 collagen 誘發 關節炎에 대한 鎭痛 效果 및 그 機轉에 관한 硏究-opioidergic mechanism을 중심으로-" 대한침구학회 23 (23): 149-162, 2006

21 류성룡, "電鍼刺戟의 Collagen 유발 關節炎 動物모델에 대한 鎭痛效果 및 機轉에 관한 硏究-Serotonergic mechanism을 중심으로-" 대한침구학회 23 (23): 77-90, 2006

22 대한침구학회 교재편찬위원회, "鍼灸學 下" 집문당 136-138, 2008

23 채충헌, "足三里 絲瓜絡藥鍼이 생쥐의 collagen-induced Arthritis에 미치는 영향" 대한침구학회 22 (22): 131-144, 2005

24 양기영, "足三里 獨活藥鍼이 collagen-induced arthritis에 미치는 영향" 대한침구학회 23 (23): 191-206, 2006

25 정영돈, "足三里 桑枝藥鍼이 Collagen-induced arthritis에 미치는 영향" 대한침구학회 23 (23): 29-44, 2006

26 巢元方, "諸病源候論" 人民衛生出版社 5-8, 1982

27 임형호, "絡石藤의 效能에 關한 實驗的 硏究" 7 (7): 334-341, 1991

28 신민교, "絡石藤과 地錦의 效能에 關한 書誌學的 比較考察" 13 (13): 37-43, 1988

29 申佶求, "申氏本草學" 수문사 546-547, 1973

30 김영화, "海桐皮藥鍼이 Collagen-induced Arthritis 억제 및 면역조절에 미치는 영향" 대한침구학회 24 (24): 171-183, 2007

31 전국한의과대학 본초학편집위원회, "本草學" 영림사 319-320, 2005

32 신동수, "木瓜 藥鍼이 Collagen-induced Arthritis 억제 및 면역조절에 미치는 영향" 대한침구학회 24 (24): 127-143, 2007

33 張景岳, "景岳全書" 한미의학 477-490, 2006

34 Harris JA, "Using c-fos as a Neural Marker of Pain" 45 (45): 1-8, 1998

35 Solodkin A, "Unilateral hindpaw inflammation produces a bilateral in- crease in NADPH-diaphorase histochemical staining in the rat lumbar spinal cord" 51 : 495-499, 1992

36 Segal B, "Tumor necrosis factor(TNF) inhibitor therapy for rheumatoid arthritis" 106 (106): 778-787, 2008

37 Hosaka K, "The combined effects of anti-TNFα antibody and IL-1 receptor antagonist in human rheumatoid arthritis synovial membrane" 32 (32): 263-269, 2005

38 Sambrook P, "The Musculoskeletal System" 1 (1): 2001

39 McCartney-Francis N, "Supression of arthritis by an inhibitor of nitric oxide synthase" 178 : 749-754, 1996

40 장선일, "Suppressive Effect of Kagam-bojungikgitang on Collagen Induced Arthritis in DBA1J Mice" 대한본초학회 18 (18): 143-151, 2003

41 Iwakura Y, "Roles of IL-1 in the development of rheumatoid arthritis: consideration from mouse models" 13 (13): 341-355, 2002

42 Bunim JJ, "Rheumatoid arthritis: A review of recent ad- vances in our knowledge concerning pathology, diagnosis, and treatment" 1 (1): 168-210, 1955

43 Klareskog L, "Rheumatoid arthritis and its animal models: the role of TNF-α and the possible absence of specific immune reac- tions" 11 (11): 657-662, 1999

44 Quintero L, "Repeated swim stress increases pain-induced expression of c-Fos in the rat lumbar cord" 965 (965): 259-268, 2003

45 Andersson AK, "Recent de- velopments in the immunobiology of rheumatoid arthritis" 10 (10): 204-, 2008

46 Glynn LE, "Pathology, pathogenesis, and aetiology of rheumatoid arthritis" 31 (31): 412-420, 1972

47 조성범, "Lidocaine에 의한 경련이 뇌조직에서 c-fos 발현에 미치는 영향" 7 (7): 89-90, 1998

48 Farrell AJ, "Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic disease" 51 : 1219-1222, 1992

49 Haas CS, "In vivo inhibition of angiogenesis by interleukin- 13 gene therapy in a rat model of rheumatoid arthritis" 56 (56): 2535-2548, 2007

50 Xu L, "In situ measurement of neuronal nitric oxide synthase activity in the spinal cord by NADPH-diaphorase histochemistry" 150 (150): 174-184, 2006

51 Trentham DE, "Humoral and cellular sensitivity to collagen in type Ⅱ collagen-induced arthritis in rats" 61 : 89-96, 1978

52 해리슨내과학 편집위원회, "Harrison’s 내과학" 도서출판 MIP 2148-2156, 2006

53 American College of Rheumatology, "Guidelines for the Management of Rheumatoid Arthritis" 46 (46): 328-346, 2002

54 British Society for Rheumatology and British Health Professionals in Rheumatology, "Guideline for the Management of Rheumatoid Arthritis (the first two years)" 45 (45): 1167-1169, 2006

55 Aloisi AM, "Gonad- ectomy and persistent pain differently affect hippocampal c-Fos expression in male and female rats" 281 (281): 29-32, 2000

56 Brennan FM, "Evidence that cytokines play a role in rheumatoid arthritis" 118 (118): 3537-3545, 2008

57 Ceccarelli I, "Effects of Formalin Pain on Hippocampal c-Fos Ex- pression in Male and Female Rats" 64 (64): 797-802, 1999

58 Myers LK, "Collagen-induced arthritis, an animal model of autoimmunity" 61 (61): 1861-1878, 1997

59 Khalifeh MS, "Clinical and histopathological evaluation of MDP/collagen induced arthritis rat model (MCIA) after treatment with Urtica dioica, plantago major and Hypericum perforatum L herbal mixture" 57 (57): 101-110, 2008

60 Augello A, "Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis" 56 (56): 1175-1186, 2007

61 Trentham DE, "Auto- immunity to type Ⅱ collagen : An experimental model of arthritis" 146 : 857-868, 1977

62 최정식, "A Study on the Effects of Spatholubus suberectus Dunn on the Inhibition of Arthritis Induced by Collagen on the Mouse" 대한본초학회 18 (18): 79-88, 2003

63 American College of Rheumatology, "2008 Recom- mendations for the Use of Nonbiologic and Biologic Disease-Modifying Antirheumatic Drugs in Rheumatoid Arthritis" 59 (59): 762-784, 2008