Porous Microcarrier-Enabled Three-Dimensional Culture of Chondrocytes for Cartilage Engineering: A Feasibility Study

다국어 초록 (Multilingual Abstract)

Cartilage repair is substantially intractable due to poor self-healing ability. Porous microspheres can be a fascinating three-dimensional matrix for cell culture and injectable carrier in cartilage engineering. In this study, we assessed the feasible use of porous biopolymer microspheres for chondrocyte carriers. When seeded onto the blended biopolymer microspheres and followed by a dynamic spinner flask culture, the chondrocytes showed robust growth behaviors during the culture period. The gene expressions of SOX9, type II collagen, and aggrecan were significantly upregulated after 2-week of culture. Furthermore, immunolocalization of type II collagen and secretion of glycosaminolglycan became prominent. The results suggest the feasible usefulness of the porous microspheres as the cell culture matrix and the subsequent delivery into cartilage defects.

참고문헌 (Reference)

1 Langer R, "Tissue engineering" 260 : 920-926, 1993

2 Malda J, "The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage" 26 : 63-72, 2005

3 Park JH, "Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration" 28 : 1079-1084, 2014

4 Lee HH, "Preparation of hydroxyapatite spheres with an internal cavity as a scaffold for hard tissue regeneration" 19 : 3029-3034, 2008

5 Kang SW, "Poly(lactic-co-glycolic acid)microspheres as an injectable scaffold for cartilage tissue engineering" 11 : 438-447, 2005

6 Diduch DR, "Marrow stromal cells embedded in alginate for repair of osteochondral defects" 16 : 571-577, 2000

7 Wang W, "In vivo restoration of full-thickness cartilage defects by poly(lactide-co-glycolide) sponges filled with fibrin gel, bone marrow mesenchymal stem cells and DNA complexes" 31 : 5953-5965, 2010

8 Chen J, "In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells" 319 : 429-438, 2005

9 Tognana E, "Hyalograft C: hyaluronan-based scaffolds in tissue-engineered cartilage" 186 : 97-103, 2007

10 Frondoza C, "Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture" 17 : 879-888, 1996

11 Ruuskanen MM, "Generation of cartilage from auricular and rib free perichondrial grafts around a self-reinforced polyglycolic acid mould in rabbits" 28 : 81-86, 1994

12 Jin GZ, "Dynamic cell culture on porous biopolymer microcarriers in a spinner flask for bone tissue engineering: a feasibility study" 36 : 1539-1548, 2014

13 Oh SA, "Collagen three-dimensional hydrogel matrix carrying basic fibroblast growth factor for the cultivation of mesenchymal stem cells and osteogenic differentiation" 18 : 1087-1100, 2012

14 Awad HA, "Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds" 25 : 3211-3222, 2004

15 Vinatier C, "Cartilage tissue engineering: towards a biomaterial-assisted mesenchymal stem cell therapy" 4 : 318-329, 2009

16 Hunziker EB, "Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects" 10 : 432-463, 2002

17 Mercier NR, "A novel injectable approach for cartilage formation in vivo using PLG microspheres" 32 : 418-429, 2004