The characteristics of integrins expression in decidualized human endometrial stromal cell induced by 8-Br-cAMP in in vitro

다국어 초록 (Multilingual Abstract)

Integrins are heterodimeric glycoproteins that havebeen found to undergo dynamic temporal and spa-tial changes in the endometrium during the men-strual cycle and in early pregnancy. Specificity ofintegrins is known to be different in human endome-trial stromal cells and decidual cells. These shifts ofintegrins suggested to play an important role inembryo implantation and can be modulated byprogesterone, cAMP derivatives, and cytokines. Themechanisms of decidualization and its precise phys-iological role are still not clearly understood and invitro systems could provide an alternative that over-comes limitations of studying such complex biolog-ical phenomena in vivo at the time of implantation.This study was undertaken to establish an in vitromodel system for human decidualization using 8-bromo-cAMP and to investigate the characteristicsof stromal integrin expression in vitro by 8-Br-cAMP.Endometrial stromal cells were isolated and cul-tured, and then were induced to decidualize by 0.5mM 8-Br-cAMP for 15 days. Immunofluorescencestaining and flow cytometric analyses of the integrinsubunits ( a1, a4, a5, a6, b1 and avb3) were per-formed at day 9. In the presence of 8-Br-cAMP, thestaining intensity of avb3 was significantly higherthan control and measurements for a1, a4, a5, a6,and b1 were similar. Immunofluorescent localizationof the integrins reflected the diferences obtainedfrom the flow cytometric analyses described above.In summary, the expresion of avb3 integrin increasedin stromal cells in vitro decidualized by 8-Br-cAMPand this up-regulation of avb3 integrin expressionduring decidualization might influence on humanimplantation.