OVA로 유도된 천식 모델 생쥐에 대한 필발의 폐포내 호산구 침윤 및 증식 억제효과 = Inhibitory Effects of Piperis Longi Fructus on the Accumulation of Eosinophils into Airways and Cell Proliferation

다국어 초록 (Multilingual Abstract)

Objective : Asthma is a chronic inflammatory disease of the mucosa and is associated with excess production of Th2 cytokines and eosinophil accumulation in lung. Piper longum L. is one of the well known herb used in oriental medicine for treatment asthma, bronchial and lung inflammation. In this study, effects of Piper lungum L. water extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and genes expression were evaluated. Anti-asthmatic effects of Piper longum L. in the development of OVA-induced airway inflammation and murine asthma model have not been fully investigated in vivo.
To clarify the possible effects of Piper longum L. we examined the influence of Piperis longi fructus(PLF) on the development of pulmonary eosinophilic inflammation in a murine model of asthma.

Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 ㎖ alum-precipitated Ag containing 100 ㎍ of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PLF (150 mg/kg) were orally administered 3 times a week for 8 weeks.

Results : The suppressive effects of PLF were demonstrated on the accumulation of eosinophills into airways, with reduction of eosinophil, total lung leukocytes numbers.

Conclusions : These results indicate that PLF has a deep inhibitory effect on airway inflammation and hyperresponsiveness in murine asthma model and that suppression of reduction of eosinophil, total lung leukocytes numbers, eosinophil CCR3 expression and CD11b expression contributes this effect. Hence, the results indicated that PLF could act as a immunomodulator which possess anti-inflammatory and anti-asthmatic property.

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