갈파래(Ulva lactuca) 추출분획의 암 세포주에 대한 세포독성 및 면역활성 효과 = Effects of Ulva lactuca Extracts on Cytotoxicity of Cancer Cell Lines and Immune Stimulation

국문 초록 (Abstract)

본 연구는 갈파래(Ulva lactuca) 추출분획의 암세포주에 대한 세포독성 및 면역활성 효과를 조사하였다. 갈파래의 에탄올 추출물로부터 분획물의 제조는 hexane, ethyl ether, methanol, butanol, $H_2O$의 용매를 이용하여 행하였다. 인간 백혈암세포주(U937), 생쥐 신경아종세포주(NB41A3), 인체간암세포주(HepG2),큰지 신경교세포주(C6) 등에 대한 갈파래 분획물의 세포독성을 측정하였다. 갈파래의 Ethyl ether 층은 4종류의 세포 모두에서 가장 높은 세포독성을 나타냈다. 또한 수층 역시 비교적 높은 세포독성을 나타냈다. 4종류의 세포 모두에서 농도의존적 경향을 나타냈다. 갈파래 분획물의 큰쥐 대식세포주(RAW 264.7)에 대한 면역활성 효과도 조사하였다. 갈파래 추출물의 5가지 분획물 모두에서 농도의존적으로 NO 생산을 활성화시켰다. 이러한 결과들로 잔파래가 항암 덴 면역활성을 나타내는 천연 소재개발에 있어 유용한 후보가 될 수 있을 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and $H_2O$. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition, $H_2O$ fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.

참고문헌 (Reference)

1 "Potent suppressive effect of a Japanese edible seaweed on initiation and promotion phases of chemically induced mouse skin tumorigenesis" 140 : 21-25, 1999

2 "Plant phenolics as potential cancer prevention agents" 401 : 25-34, 1996

3 "Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay" 589-595, 1988

4 "Evalution of a tetrazolium based semiautomated colorimetric assay assessment of chemosensitivity testing" 936-942, 1987

5 "Effects of verapamil tamoxifen and cyclosporin A for the modulation of multidrug resistance in human lung cancer cell lines" 225-230, 1993

6 "Determination of Na channel blocker in seaweeds" 19 : 107-112, 2001

7 "Desmutagenic effects of extract obtained from seaweeds" 502-508, 1986

8 "Cancer-protective factor in fruit and vegetable" 116-135, 1993

9 "Cancer Causes Aontril" 427-442, 1991

10 "Antiumor polysaccharide fraction from Sargassume thunbergii" 114-115, 1976

11 "Antimutagenic and anticarcinogenic effects of carotenoids and dietary palm oil" 287-295, 1992

12 "Anticomplementary activities of rhamman sulfate extrated from Monastraomsa nitidum" 4 : 490-495, 1996