This study investigates survival and development in vitro of micromanipulated embryos and biopsied blastomeres during early development of mouse embryos. The micromanipulated 4-cell and intact 4-cell embryos in Medium 2 developed to blastocyst stage b...
This study investigates survival and development in vitro of micromanipulated embryos and biopsied blastomeres during early development of mouse embryos. The micromanipulated 4-cell and intact 4-cell embryos in Medium 2 developed to blastocyst stage by 83.3% and 90.4%, respectively. Blastomeres either biopsies or separates of 4-cell embryos in vitro developed to trophoblastic vesicle by 80.8% and 83.3%, respectively. After transfer to pseudopregnant recipient mice, the offspring rates of biopsied embryos and intact embryos were 36% and 48.6% respectively. The results suggest that the biopsy of single blastomeres from 4-cell mouse embryos have no detrimental effect on survival of embryos and blastomeres.