Agrobacterium Mediated Transformation of Nicotiana glauca, a Woody Plant

다국어 초록 (Multilingual Abstract)

Nicotiana glauca is a fast growing perennial and bears thousands of seed every year. These characteristics could provide advantages of using N. glauca as a model system for the study of gene expression controls in woody plants. Leaf explants of N. glauca were cocultured with Agrobacterium tumefaciens strain LBA4404 carrying a plasmid pBI121 and in vitro regenerated. Regenerating cells showed strong resistance to kanamycin on the in vitro regeneration medium, and transformants were efficiently selected on the medium containing 300 mg/l of kanamycin. In vitro regenerated N. glauca plants showed specific northern signals for β-glucuronidase transcripts. The seeds obtained from the self-fertilized transformed plants showed a Mendelian pattern of inheritance in kanamycin-resistance. T_2 generation plants from the kanamycin-resistant seeds also showed specific northern signals for β-glucuronidase transcripts.