LncRNA PVT1 upregulates FBN1 by sponging miR-30b-5p to aggravate pulpitis

다국어 초록 (Multilingual Abstract)

Background Pulpitis is a disease mainly caused by bacterial infection. Long noncoding RNAs (lncRNAs) infl uence the advancement of pulpitis. In this paper, we reconnoitered the characters of lncRNA plasmacytoma variant translocation 1 (PVT1) in pulpitis.
Objective PVT1, microRNA-30b-5p (miR-30b-5p) and fi brillin 1 (FBN1) levels were identifi ed by qRT-PCR and western blot. In addition, the cell functions were scrutinized. Additionally, the link between miR-30b-5p and PVT1 or FBN1 was identifi ed by dual-luciferase reporter assay.
Result The contents of PVT1 and FBN1 were augmented, but the miR-30b-5p level was declined in pulpitis. Knockdown of PVT1 enhanced cell proliferation and cell vitality, whereas inhibited cell infl ammatory reaction in HDPFs under LPS stimulation. In mechanism, PVT1 acted as a miR-30b-5p sponge to adjust the content of FBN1. Moreover, miR-30b-5p mediated LPS-mediated repression of proliferation and promotion of infl ammatory reaction HDPFs by FBN1.
Conclusion PVT1 expedited the improvement of pulpitis via miR-30b-5p/FBN1.