Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells

다국어 초록 (Multilingual Abstract)

We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, a- and b-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of 80 mM, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with a- and b-eudesmol of 80 mM. Thus, both a- and b-eudesmol exerted caspase-3 activation and cytochrome c release at 80 mM in HL-60 cells. These results are firstly reported that the sesquiterpenes, a- and b-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.

참고문헌 (Reference)

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