으름덩굴 추출물 처리에 의한 꿀벌 노제마병 감염 억제 = Prevention of Nosema Infection in Trichoplusia ni Cell Line Using Akebia quinata Extract

다국어 초록 (Multilingual Abstract)

Nosemosis is a honeybee ( Apis mellifera) disease resulting from the intake of Nosema ceranae, which recently became a major threat to honeybees. N. ceranae is a microsporidian, which acts like a parasite and remains as a spore until the infection, resulting in high management difficulty.
Until today, cure and preventative candidates are barely established. In this study, the preventive activity of Akebia quinata extract against nosemosis was done using Trichoplusia ni cell line, BTI-TN5B1-4, as a substitute for the honey bee cell line. Normal T. ni cells exhibited a normal size with round shape. However, after N. ceranae were treated, some cells were abnormally enlarged due to N. ceranae infection. Also, a nucleus of those cells was barely observable under microscope. When A. quinata extract was treated with N. ceranae, those cells did not show such symptoms and most N. ceranae spores were observed outside of the cells. For practical use of A. quinata for nosemosis, an adequate concentration range to prevent nosemosis infection was determined. The lowest concentration where A. quinata had preventative effect was set as a minimum concentration and the concentration which A. quinata extracts had cytotoxicity in the insect cells was set as maximum concentration.