Hemodynamic forces are essential to maintain the innate characters of vascular endothelial cell (VEC) expressed in its morphological and physiological behavior. The objective of this study is to investigate the effects of uniform shear stress on the d...
Hemodynamic forces are essential to maintain the innate characters of vascular endothelial cell (VEC) expressed in its morphological and physiological behavior. The objective of this study is to investigate the effects of uniform shear stress on the dynamic responses of VECs in vitro. By perfusing the growth media through a parallel plate flow chamber which is developed in three-dimensional computational fluid dynamics analysis, we can observe the dynamic behavior of cells in real time for a long term period. Under various flow conditions, the shear stress effects on the cell motility and proliferation rate in the mouse micro-vessel endothelial cell are studied. Surprisingly, we observe that most cells show the tendency to migrate against the flow direction independently of the magnitude of shear stress. Moreover, we examine that proliferation rate is decreased as the shear stress magnitude increases. The dynamic responses of VECs such as cell migration and proliferation rate exhibit significant changes depending on the magnitude of shear stress.