Neuroprotective effect of Allium hookeri against H2O2-induced PC12 cell cytotoxicity by reducing oxidative stress

다국어 초록 (Multilingual Abstract)

In this study, the anti-oxidative and neuro-protectiveeffects of ethanolic extracts of the dried roots ofAllium hookeri were investigated. Total phenolic contentsand total flavonoid contents of A. hookeri extract dependedon the ethanol concentrations used (50, 70 and 95%). Inorder to evaluate radical scavenging activity, DPPH andABTS radical scavenging assays and ferric reducingpowers were evaluated. The results showed the 95%ethanol extract of A. hookeri (95AH) had higher phenolicand flavonoid contents, and greater radical scavengingactivities than 50 or 70% ethanol extracts of A. hookeri.
The neuro-protective effects of 95AH were evaluated usingH2O2-treated PC12 neuronal cells. Treatment of 95AHincreased cell viability and superoxide dismutase and glutathioneperoxidase activities, reduced lactate dehydrogenaterelease, reduced reactive oxygen speciesproduction, and increased Bcl-2/Bax ratio. HPLC revealed95AH was rich in phenolics, especially catechin. Theseresults demonstrate 95AH has substantial anti-oxidativeand neuro-protective effects against H2O2-induced oxidativestress.

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