꾸지뽕나무의 ITS 영역 염기서열 분석에 의한 유연관계 분석 = Phylogenetic Analysis of Cudrania tricuspidata using DNA Sequences of Nuclear Ribosomal ITS Region

다국어 초록 (Multilingual Abstract)

The comparison of the internal transcribed spacer (ITS) sequences of nuclear rDNA of 8 distingushed male and female of Cudrania tricuspidata (Call.) Bureau were made for the genetic analysis. The length of total nuclear DNA was showed 717 nt and including 231 nt of ITS1 gene, 158 nt of 5.8S gene and 239 nt of ITS2 gene. Based on the sequence analysis of amplified ITS gene region, the homologies were showed 100% from male and female of Naju, male of Jinu, Hongseong, Boryeong and female of Goheung plant. However, these palnts showed 99.8% and 99.4% homologies with those of female of Gunsan, male of Sunchang and female of Sunchang plants, at nucleotide level, respectively. The female and male of Imsil plants showed 99.8% homologies. The homologies between Cudrania tricuspidata and Molrus alba (Genebank accession number FJ599759) palnts showed 66.7%. The results suggested that Cudrania tricuspidata were distinct from Molrus alba and showed very close relationship between Cudrania tricuspidata plants at the nucleotide level of ITS region.