Efficient decolorization and detoxification of textile industry effluent by <i>Salvinia molesta</i> in lagoon treatment

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P> <I>Salvinia molesta</I>, an aquatic fern was observed to have a potential of degrading azo dye Rubine GFL up to 97% at a concentration of 100mg/L within 72h using 60±2g of root biomass. Both root as well as stem tissues showed induction in activities of the enzymes such as lignin peroxidase, veratryl alcohol oxidase, laccase, tyrosinase, catalase, DCIP reductase and superoxide dismutase during decolorization of Rubine GFL. FTIR, GC-MS, HPLC and UV–visible spectrophotometric analysis confirmed phytotransformation of the model dye into smaller molecules. Analysis of metabolites revealed breakdown of an azo bond of Rubine GFL by the action of lignin peroxidase and laccase and formation of 2-methyl-4-nitroaniline and N-methylbenzene-1, 4-diamine. Anatomical tracing of dye in the stem of <I>S. molesta</I> confirmed the presence of dye in tissues and subsequent removal after 48h of treatment. The concentration of chlorophyll pigments like chlorophyll a, chlorophyll b and carotenoid was observed during the treatment. Toxicity analysis on seeds of <I>Triticum aestivum</I> and <I>Phaseolus mungo</I> revealed the decreased toxicity of dye metabolites. <I>In situ</I> treatment of a real textile effluent was further monitored in a constructed lagoon of the dimensions of 7m×5m×2m (total surface area 35m<SUP>2</SUP>) using <I>S. molesta</I> for 192h. This large scale treatment was found to significantly reduce the values of COD, BOD<SUB>5</SUB> and ADMI by 76%, 82% and 81% considering initial values 1185, 1440mg/L and 950 units, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Macrophyte <I>S. molesta</I> showed a potential for textile dyes and effluent treatment </LI> <LI> A possible dye degradation pathway of Rubine GFL by <I>S. molesta</I> is proposed </LI> <LI> <I>S. molesta</I> in constructed lagoon treated 52,500L of textile effluent </LI> <LI> Phytotoxicity assay revealed less toxic nature of by-products after treatment </LI> <LI> Anatomical study of stem revealed entry and removal of Rubine GFL </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>