This study was to stimulate β-galactosidase production of Bacillus coagulans ATCC8038, a thermophilic, sporeforming, and facultatively anaerobic lactose fermenting bacterium.
Optimum initial pH for the enzyme production was pH 7.0 in the medium whic...
This study was to stimulate β-galactosidase production of Bacillus coagulans ATCC8038, a thermophilic, sporeforming, and facultatively anaerobic lactose fermenting bacterium.
Optimum initial pH for the enzyme production was pH 7.0 in the medium which contained 0.4% of lactose, 0.1% of peptone, 0.2 % of yeast extract, 0.7% Na-acetate. 3 H₂O and 0.03% CaCl₂.2H₂O. Potassium phospate buffer(0.05M, pH 7.0)induced the better production of β- galactosidase, and optimum concentrations of lactose, yemit extract, peptone and Na - acetate. 3H₂O in β-galactosidase production medium were found to be 0.4% , 1%, 0.1% and 0.7% respectively.