Physicochemical and antibacterial characterization of Aspergillus sp. filtrate-reduced graphene oxide

다국어 초록 (Multilingual Abstract)

Graphene-based nanomaterials are rapidly emerging as antimicrobial agents against a range of pathogenicmicroorganisms. In the present study, we have demonstrated the synthesis of reduced grapheneoxide (RGO) using Aspergillus sp. filtrate as a reducing agent. The obtained Aspergillus sp. filtrate-RGO(AF-RGO) was characterized by ultraviolet–visible absorption spectroscopy, dynamic light scattering,X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, scanning electronmicroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and atomic force spectroscopyto obtain details about the size, composition, structure, and morphology. The characterizationconfirmed the reduction of graphene oxide, the restoration of sp2 conjugation, and the formation offew-layered RGO sheets. The surface charge and stability of the AF-RGO in aqueous media were examinedby zeta potential measurements. The thermal stability of AF-RGO was increased significantly due to therestoration of the graphitic sp2 network. The antibacterial activity of AF-RGO was evaluated using theagar well diffusion method, broth microdilution method, and Live/Dead bacterial viability assay. AFRGOexhibited good antibacterial activities against pathogenic Gram-negative (Escherichia coli) andGram-positive (Staphylococcus aureus) bacteria with minimum inhibitory concentration (MIC) and minimumbactericidal concentration (MBC) values of 15 and 30 mg/ml, respectively. Therefore, fungal filtratemediatedsynthesis of RGO is a promising antibacterial carbon nanomaterial for a range of biomedical andnano(bio)technological applications.

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