PCR-DGGE and Real-time PCR dsrB-based Study of the Impact of Heavy Metals on the Diversity and Abundance of Sulfate-reducing Bacteria

다국어 초록 (Multilingual Abstract)

Sulfate-reducing bacteria (SRB) are widelyused for heavy metal (HM) treatment in bioreactors buttheir growth and biological activity can be inhibited bysuch treatment. Here, bioreactor experiments were used toinvestigate changes in the SRB community and the copynumber of the dissimilatory sulfite reductase β-subunitfunctional gene (dsrB) under high doses of sulfates andHMs. The SRB community was investigated using polymerasechain reaction denaturing gradient gel electrophoresis(PCR-DGGE) and sequencing techniques, while the dsrBgene abundance was measured by quantitative real-timePCR (qRT-PCR). The sulfate reduction rate was initiallymuch higher in reactors without HMs than in thosecontaining HMs (p = 0.001). Sulfate levels were reducedby 50% within the first 3 days of operation. As a result, theHM removal rate was initially much lower in the reactorscontaining HMs. Most of the HMs reduced to safe limitswithin 9 ~ 12 days of operation. The SRB communitymainly consisted of Desulfovibrio vulgaris, D. termitidis,D. desulfuricans, D. simplex and Desulfomicrobium baculatum,as determined by PCR-DGGE. qRT-PCR revealed adecreasing trend in the copy numbers of a functional gene(dsrB) after 6 days in samples lacking HMs; however, theopposite trend was observed in the HM-containing samples.

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