산겨릅나무(Acer tegmentosum Max.)의 액아배양으로부터 다신초 형성을 통한 대량증식 = Micropropagation of Acer tegmentosum Max. through induction of multiple shoots from axillary bud cultures

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the optimal conditions for in vitro culture of Acertegmentosum Max(Aceraceae), a medicinal plant. For mass propagation, the optimal medium andplant growth regulators for rooting, and conditions for hardening were established. Seeds weresterilized, and then germinated on MS medium containing 1.0mg l-1 GA3 for obtaining in vitroplantlets. To investigate the optimal culture medium, the plantles which were inoculated inWPM media showed the best growths. The largest number of multiple shoots from axillary budswere obtained in the treatment of 1.0mg l-1 Zeatin(3.3 shoots per a stem segment). Highnumbers of roots were developed in the treatments of 0.5mg l-1 2.4.-D. The plantlings weretransferred into soil to be adapted to in vivo condition. These results can contribute forpropagating A. tegmentosum.

국문 초록 (Abstract)

산겨릅나무(Acer tegmentosum Max)는 단풍나무과의 약용식물로 기내 대량증식 및 발근에 적합한최적 배지와 식물생장조절물질의 농도를 구명하고, 순화조건을 검증하여 대량생산조건을 확립하기 위해수행되었다. 산겨릅나무의 종자 소독 후 1.0mg l-1 GA3가 첨가된 MS배지에서 발아시켜 기내식물체를확보하였고, 생장에 적합한 최적배지 구명을 위해 7종류의 다양한 식물배지에서 4주간 배양한 결과,WPM 배지가 가장 효과적이었다. 액아배양을 통한 대량증식에 최적 조건을 구명하기 위하여 4종의cytokinin의 다양한 농도별 처리를 통해서 8주 동안 배양한 결과, 1.0mg l-1 Zeatin이 첨가된 배지에서평균 3.3개의 다경줄기가 유도되었다. 기내발근에는 0.5mg l-1 2.4.-D가 단독 첨가된 배지에서 뿌리수가 높았고, 다신초 개체는 기외의 토양으로 이식하여 환경적응을 유도한 후 배양묘 대량생산 조건을확립하였다.

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