DNA 마커를 이용한 야생 복숭아 유전자원의 유전적 다양성 분석

국문 초록 (Abstract)

본 연구는 복숭아 유전자원의 유전적 다양성을 분석하여 육종의 기초 자료로 활용하기 위하여 재배 품종 및 국내에서 수집한 야생 복숭아 64점을 대상으로 SRAP, AFLP와 RAPD 분석을 수행하였다. 19종과 20종의 primer조합을 이용한 SRAP와 AFLP분석에서 각각 143개(26.7%)와 193개(27.2%)의 다형성 밴드를 획득하였고, 평균 다형성 밴드 수는 7.53개와 9.65개였다. RAPD분석에서 52종의 선발 primer를 이용하여 201개(45.9%)의 다형성 밴드를 얻었으며, 평균 다형성 밴드 수는 3.90개였다. SRAP, AFLP와 RAPD분석에서 획득된 537개의 다형성 밴드를 이용하여 UPGMA(비가중 평균결합) 방식으로 유사도 및 집괴분석을 수행한 결과 유전적 유사도 0.751을 기준으로 8개의 그룹으로 분류되었다. 복숭아 유전자원 간 유전적 유사도 값은 0.370～0.978의 범위로 평균 유전적 유사도는 0.737이었다. 가장 높은 유사도 값(0.978)을 나타낸 유전자원은 PH065와 PH066 간이었고 가장 낮은 유사도 값(0.370)을 나타낸 유전자원은 Prunus davidiana와 PH020간이었다. 본 연구에서는 이용된 64종의 복숭아 유전자원은 집괴분석 결과 유전자원들이 수집된 지역은 다르지만 유전적 다양성은 낮은 것으로 추정되었다.

다국어 초록 (Multilingual Abstract)

In this study, sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and randomly amplified polymorphic DNA (RAPD) analyses were used to assess genetic diversity among 64 peach germplasms including commercial cultivars and wild accessions. Nineteen SRAP primer combinations were detected a total of 143 polymorphic bands (26.7%) with an average 7.53. In AFLP analysis, twenty primer combinations were generated a total of 193 polymorphic bands (27.2%) with an average 9.65. Fifty-two RAPD primers were detected a total of 201 polymorphic bands (45.9%) with an average of 3.90. By UPGMA (unweighted pair-group method arithmetic average) cluster analysis with 537 polymorphic bands, the peach germplasms were classified eight groups by similarity index of 0.751. Genetic similarity values among the tested peach germplasms ranged from 0.370 to 0.978, and the average similarity value was 0.737. The similarity index was the highest (0.978) between PH065 and PH066, and the lowest (0.370) between Prunus davidiana and PH020. The genetic diversity among the 64 peach germplasms revealed by cluster analysis was low, despite they were collected from different regions.

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