Nickel Increases Chemotactic Activity of Porcine Peripheral Blood Polymorphonuclear Cells

다국어 초록 (Multilingual Abstract)

Nickel is a nutritionally essential trace element that plays an important role in the immune system of several animal species. The aim of this study was to examine the effect of nickel chloride on chemotactic activity of peripheral blood polymorphonuclear cells (PMNs) and whether this effect is associated with interleukin (IL)-8 and a nuclear factorkappa B (NF-B)-dependent pathway. Peripheral blood mononuclear cells (PBMCs) and PMNs were isolated by Percoll solution (Specific gravity; 1.080) and 1.5% dextran treatment, respectively. A modified Boyden chamber assay was used to measure the chemotactic activity of PMNs. The level of IL-8 in culture supernatant from PBMCs was measured by enzyme-linked immunosorbent assay (ELISA). Both of PBMCs and PMNs exhibited a low viability when cultured with concentration of greater than 1,000 M of nickel chloride for 24 h. Thus, nickel chloride was used at concentration of 500 M, which preserved cell viability. Treatment with nickel did not directly affect the chemotactic activity of PMNs. However, the chemotactic activity of PMNs was remarkably increased by culture supernatant from PBMCs treated with nickel chloride (500 M) for 24 h. Recombinant porcine IL-8 polyclonal antibody (pAb) neutralized the enhancing effect on the chemotactic activity of PMNs by culture supernatant from PBMCs treated with nickel and this culture supernatant had higher IL-8 levels than the culture supernatant from untreated PBMCs. In addition, n-tosyll- phenylalanine chloromethyl ketone (TPCK), a NF-B inhibitor, antagonized the enhancing effect on the chemotactic activity of PMNs by the culture supernatant from PBMCs treated with nickel. These results suggested that nickel stimulates porcine PBMCs to produce IL-8, which increases the chemotaxis of PMNs via NF-B-dependent pathway.

참고문헌 (Reference)

1 Freitas M, "Zinc, cadmium and nickel increase the activation of NF-κB and the release of cytokines from THP-1 monocytic cells" 3 : 1238-1243, 2011

2 Strober W, "Trypan blue exclusion test of cell viability" 111 : A3.B.1-A3.B.3, 2015

3 O'Flaherty JT, "Substances which aggregate neutrophils. Mechanism of action" 92 : 155-166, 1978

4 Hunt JA, "Stimulation of neutrophil movement by metal ions" 26 : 819-828, 1992

5 Kreisle RA, "Specific binding of leukotriene B4 to a receptor on human polymorphonuclear leukocytes" 157 : 628-641, 1983

6 Haase H, "Signal transduction in monocytes : the role of zinc ions" 20 : 579-585, 2007

7 Kharazmi A, "Recombinant interleukin α and β prime human monocyte superoxide production but gave no effect on chemotaxis and oxidative burst response of neutrophils" 177 : 32-39, 1988

8 Sydor AM, "Nickel metallomics : general themes guiding nickel homeostasis" 12 : 375-416, 2013

9 Freitas M, "Nickel induces oxidative burst, NF-κB activation and interleukin-8 production in human neutrophils" 15 : 1275-1283, 2010

10 Guo H, "Nickel chloride (NiCl2)-caused inflammatory responses via activation of NF-κB pathway and reduction of anti-inflammatory mediator expression in the kidney" 6 : 28607-28620, 2015

11 Spears JW, "Nickel as a "newer trace element" in the nutrition of domestic animals" 59 : 823-835, 1984

12 Baggiolini M, "Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils" 84 : 1045-1049, 1989

13 Kolaczkowska E, "Neutrophil recruitment and function in health and inflammation" 13 : 159-175, 2013

14 White MV, "Neutrophil attractant/activation protein-1(NAP-1)causes human basophil histamine release" 22 : 151-154, 1989

15 Gloire G, "NF-kappaB activation by reactive oxygen species : fifteen years later" 72 : 1493-1505, 2006

16 Tak PP, "NF-kappaB : a key role in inflammatory diseases" 107 : 7-11, 2001

17 Matsushima K, "Molecular cloning of a human monocyte-derived neutrophil chemotactic factor(MDNCF)and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor" 167 : 1883-1893, 1988

18 Szatmary AC, "Modeling neutrophil migration in dynamic chemoattractant gradients : assessing the role of exosomes during signal relay" 28 : 3457-3470, 2017

19 Schratzberger P, "Interleukin-8-induced human peripheral blood B-lymphocyte chemotaxis in vitro" 58 : 167-170, 1997

20 Baggiolini M, "Interleukin-8, a chemotactic and inflammatory cytokine" 307 : 97-101, 1992

21 Baggiolini M, "Interleukin-8 and the chemokine family" 17 : 103-108, 1995

22 Son SM, "Induction of interleukin-8 expression in porcine peripheral blood mononuclear cells by trans10-cis12 conjugated linoleic acid" 112 : 284-289, 2006

23 Hoch RC, "In vivo, in vitro, and molecular aspects of interleukin-8 and the interleukin-8receptors" 128 : 134-145, 1996

24 Bashir S, "Granulocyte concentrates : how can we assess their quality?" 13 : 245-257, 2003

25 김수현, "Fucoidan Increases Phagocytic Capacity and Oxidative Burst Activity of Canine Peripheral Blood Polymorphonuclear Cells Through TNF-α from Peripheral Blood Mononulear Cells" 한국임상수의학회 28 (28): 183-189, 2011

26 Thornton AJ, "Cytokine-induced gene expression of a neutrophil chemotactic factor/IL-8 in human hepatocytes" 144 : 2609-2613, 1990

27 Barchowsky A, "A novel pathway for nickel-induced interleukin-8 expression" 277 : 24225-24231, 2002

28 Cai T, "A cross-talk between NFAT and NF-κB pathways is crucial for nickelinduced COX-2 expression in Beas-2B cells" 11 : 548-559, 2011