C1 gas has been highlighted for biorefinery process, and it is a valuable feedstock to produce various platform chemicals. The acetate is an intermediate chemical which can be synthesized via Wood-Ljundahl pathway, and further substrate for chain elon...
C1 gas has been highlighted for biorefinery process, and it is a valuable feedstock to produce various platform chemicals. The acetate is an intermediate chemical which can be synthesized via Wood-Ljundahl pathway, and further substrate for chain elongation into a higher VFA when sufficient reducing power provided. Hydrogen gas and reducing sugar have problem for providing reducing power for C1 gas conversion (e.g. high price and catabolite repression). ZVI has been widely used as a reducing agent in environmental process. In this study, we examined that the externally provided reducing power from ZVI oxidation, increase acetate production from CO and further synthesis of volatile fatty acids. The effect ZVI on CO/CO<sub>2</sub> conversion was evaluated with acetate production and byproduct formation. The carbon and electron balance provide an information for mechanism of C1 gas conversion and chain elongation. We have shown that ZVI can provide reducing power to microbial C1 gas conversion.