Stem cell injection to skin wound site is a promising approach for wound healing. Trypsin treatment for detaching cells accompanies loss of extracellular matrix and often cause cell damage. In this study we synthesized novel pH-responsive polymer base...
Stem cell injection to skin wound site is a promising approach for wound healing. Trypsin treatment for detaching cells accompanies loss of extracellular matrix and often cause cell damage. In this study we synthesized novel pH-responsive polymer based on poly(amino ester) (mPAE) which can provide the appropriate cell culture condition for cell growth without cytotoxicity and detaching using low pH (6.0) PBS. mPAE showed no cytotoxicity and detached the human adipose derive stem cells (hADSCs) from the mPAE coated tissue culture plates (TCPs) by low pH (6.0) PBS. hADSCs cultured on and detached from mPAE coated TCPs showed similar cell adhesion and angiogenic paracrine factor secretion compared to hADSCs cultured on and detached from TCPs. Finally, hADSCs detached from mPAE coated TCPs showed similar angiogenesis and wound regeneration compared to hADSCs detached from TCPs. In conclusion, our mPAE might be utilized for enzyme free cell detachment system for future tissue engineering.