The activity of Ceruloplasmin(EC 1.16.3.1, CP) in human plasma was measured in several conditions in order to study the stability of CP. The CP was partially purified by polyethyleneglycol 4000 and Sephadex G-200 chromatography. And the Cp was determi...
The activity of Ceruloplasmin(EC 1.16.3.1, CP) in human plasma was measured in several conditions in order to study the stability of CP. The CP was partially purified by polyethyleneglycol 4000 and Sephadex G-200 chromatography. And the Cp was determined using a polyacrylamide gel electrophoresis with o-dianisidine stain. The activity of CP in human plasma was stabilized for 10days at 4 ℃ and frozen plasma was stabilized for 15days at -35℃ . The activity of CP was more stabilized in 0.05M sodium acetate buffer(pH 5.3) than 0.075M potassium phosphate buffer(pH 6.5). In addition, the activity of CP was stabilized in 0.05M sodium acetate buffer(pH 5.3) containing 0.225M NaCl.