Hydrogen peroxide에 의해 유도된 남성 생식 세포 GC-1 cell에 미치는 五子衍宗丸의 효과 연구 = Study of Ojayeonjonghwan on hydrogen peroxide-induced oxidative stress in male reproductive GC-1 germ cell lines

다국어 초록 (Multilingual Abstract)

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines.
Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition.
Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 μg/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 μg/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 μg/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed.
Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.

참고문헌 (Reference)

1 Kim SH, "The antioxidant activity of Woogyuyeum on leydig TM3 cell" Kyung Hee University Graduate School 2010

2 Chung IM, "The Effects of "Ojayeonjonghwan" on the Secretion of Sex hormones and the Antifatigue in Rats" 3 (3): 16-231, 1998

3 Kim GS, "Studying documents & papers on the cure of male sterility" 3 : 151-162, 1994

4 Ri C., "Singyopyeonjuuihagimmun" Daeseongmunhwasa 568-, 1996

5 Mosmann T., "Rapid colorimetric assay for cellular growth and survival, application to proliferation and cytotoxicity assays" 65 (65): 55-63, 1983

6 배웅진, "Protective Effects of KH-204 in the Bladder of Androgen-Deprived Rats" 대한남성과학회 33 (33): 73-80, 2015

7 김수진, "Preliminary Report on the Safety of a New Herbal Formula and Its Effect on Sperm Quality" 대한남성과학회 31 (31): 254-261, 2013

8 Namgyeongjunguihagwon, "Junguibangjedaesajeon(2)" Inminwisaengchulpansa 415-, 1994

9 Jiwon Yoon, "Effects of Ojayeonjong-hwan on cyclophosphamide-induced reproductive toxicity in male mice" 경희대학교 대학원 2013

10 Bae WJ, "Effects of KH-204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat models" 14 : 367-, 2014

11 Kim KH, "Effect of Ojayeonjonghwan on Antioxidant Capacity in D-galactose Induced Aging Rats" 09 (09): 49-63, 2005

12 Huh J., "Donguibogam" Namsandang 603-, 1994

13 Ju D, "Dangyesimbeop(Buyeo)" Daeseongmunhwasa 763-770, 1990

14 Tanaka N, "Antioxidative capacity of extracts and constituents in Cornus capitata adventitious roots" 51 (51): 5906-5910, 2003

15 Choi JH, "Antioxidant effect of Palmijihwangtang on male reproductive cells" Kyung Hee University Graduate School 2010

16 김규엽, "Anti-inflammatory effects of Ojayeonjonghwan and its component, Gomisin-A" 경희대학교 대학원 2010

17 Yagi K., "Active oxygens, lipid peroxides and antioxidants" CRC Press 1-38, 1993

18 Bradfor MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-254, 1976