SSR마커를 이용한 재배국화의 품종 판별

국문 초록 (Abstract)

본 연구는 국내에서 육성 또는 도입된 재배국화 85점을 4 개의 분자마커(SSR marker)를 이용해 유전자형을 분석하여 품 종판별에 대한 활용성을 검토하고, 유전관계를 분석하여 국화 품종 육성에 필요한 기초자료로 제공하기위해 본 실험을 수행 하였으며 결과를 요약하면 다음과 같다 1. 85점의 재배국화에 대하여 4개의 SSR 마커로 총 95개의 대립인자가 관찰되었다. 대립인자의 수는 11개 (KNUCRY-35) 에서 34개(KNUCRY-84)로 비교적 다양하게 나타났으며, 평균 대립인자 수는 23.8개였다. 유전적 다양성을 나타내는 gene diversity (GD)와 PIC 값의 범위는 0.81-0.89, 0.79-0.89로 관 찰되었다 2. 국화의 이용에 따라 재배국화를 관상국, 스탠다드국, 스 프레이국, 분화국, 화단국으로 그룹을 나눠 집단간의 유전적 다양성을 분석하였다. 각 집단의 평균 expected heterozygosity (H)와 PIC 값을 비교하였을 때 화단국이 0.25와 0.42로 가장 낮 게 나타났고 스프레이국이 0.80과 0.79로 가장 높게 나타났다. 3. SSR마커 4개 중 allele수, 특이적 allele 수, gene diversity 및 PIC 값이 높은 마커 KNUCRY-84, KNUCRY-98, KNUCRY- 77, KNUCRY-35를 단계별로 이용하여 단계별 phylogenetic tree를 작성하여 재배국화 85점을 모두 판별하였다.

다국어 초록 (Multilingual Abstract)

With increasing numbers of cultivars with variable phenotypes and a limited number of conventional ways to identify the character, cultivar complex can occur and so, plant resources should be registered genetically. The aim of this study was to develop a technique of identification using simple sequence repeat (SSR) markers for Chrysanthemum morifolium Ramat. for the purpose of plant variety protection. Eighty five cultivated chrysanthemum cultivars were assessed by four SSR markers. Four SSR markers generated a total of 95 alleles. The average number of alleles per SSR marker was 23.8 with range from 11 (KNUCRY-35) to 34 (KNUCRY-84). Gene diversity ranged from 0.81 to 0.89 with a mean of 0.84 and polymorphic information content varied from 0.79 to 0.89 with the mean of 0.82. The highest value of GD and PIC were observed at KNUCRY-35. Four markers (KNUCRY-84, KNUCRY- 98, KNUCRYCRY-77 and KNUCRY-35) were selected as specific markers for variety discrimination in chrysanthemum cultivars. All of 85 chrysanthemum cultivars were individually identified by the combination of four SSR markers.

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