N-methyl-N'-nitro-N-nitrosoguanidine에 의한 인체폐암세포의 세포사멸에 미치는 해양심층수의 영향 = Effects of Deep-sea Water on Apoptosis by N-methyl-N'-nitro-N-nitrosoguanidine in Human Lung Cancer Cells

다국어 초록 (Multilingual Abstract)

Deep-sea water (DSW) refers to sea water from a depth of more than 200 meter and is rich in minerals,
e.g., Mg, Ca, and K, which have been considered to be associated with prevention and treatment of human
diseases. In the present study, we investigated the effect of DSW on apoptotic activity by N-methyl-
N'-nitro-N-nitrosoguanidine (MNNG), a monofunctional alkylating agent, in various human cancer cell
lines. It was found that MNNG could inhibit the cell growth in a dose-dependent manner, which was
associated with dendrite-like morphological change and induction of apoptotic cell death. However, the
cells grown in medium containing DSW showed more cytotoxic and apoptotic effects than cells grown
in normal medium in A549 lung and AGS gastric cancer cell lines. The increased cytotoxic and apoptotic
effects by DSW in A549 cells was associated with the down-regulation of anti-apoptotic Bcl-XL and
cIAP-2, and cyclooxygenase-2 expression. Furthermore, the levels of cyclin-dependent kinase inhibitor
p21 (WAF1/CIP1) induced by MNNG were enhanced by DSW in a p53-independent manner. Our results
suggest a potential role for DSW as anti-apoptotic agents by enhancing apoptosis mediated through the
down-regulated expression of Bcl-XL and cIAP-2 and up-regulated expression of p21.

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