The changes of free amino acids throughout the formation of flower organs and seeds of two ginseng species, Panax quinquefolium (PQ) and Panax japonicus (PJ), were investigated and the changes in these two species were compared with the changes in Pan...
The changes of free amino acids throughout the formation of flower organs and seeds of two ginseng species, Panax quinquefolium (PQ) and Panax japonicus (PJ), were investigated and the changes in these two species were compared with the changes in Panax ginseng. As a result, some common characteristics of the ginseng plant, some characteristics of each Ginseng species, and the relation between the sterility of ginseng hybrid and metabolism of amino acids were studied. The results are summarized as follows: 1. Depending upon the species of ginseng and their growth stage, some variations in the quantity and number of amino acids were recognized. 2. In all, 19 kinds of ninhydrin sensitive substances including two amides were detected from PQ. The identified amino acids and amides were: alanine, valine, valine, leucine, phenylalanine, proline, hydroxyproline, serine, threonine, histidine, tyrosine, aspartic acid, glutamic ac id, lysine, γ-amino butyric acid, arginine, cysteic acid, tryptophan, asparagine and glutamine. From PJ, 21 kinds of ninhydrin sensitive substances were detected. Compared with PQ, β-alanine, pipecolic acid and one unknown substance were detected while histidine was not. 3. After flowering time γ-amino butyric acid was detected in PQ a nd PJ. Through the formation of the flower organ and seed of PQ and PJ, β-alanine, lysine and arginine were dected in smaller amounts than in PG. 4. The change of praline in PQ and PJ was similar to that in PG. At the microspore stage, pollen mature stage and flowering time it was detected in large amounts (+~+++) but after the falling of the petals it was hardly detected. Therfore, proline could be presumed to be closely related to the formation of the flower organ of the ginseng plant. 5. As in PG, alanine, aspartic acid, glutamic acid and cysteic acid were generally the main amino acids while valine, leucine and tyrosine appeared as comparatively distinct spots. This phenomenon seems to be common in all species of the ginseng plant. 6. Since the sulfur-containing amino acids, cysteine and cystine, are scarce in the reproductive organ of other plants, it was interesting that cysteine and cystine were always detected in large quantity in the form of cysteic acid through the formation of ginseng seeds and during the stratification period. To discover whether these sulfur containing amino acids are related to the formation of ginseng seeds and to embryo development, further studies are required.