Anticancer Activity of Marine Sponge <i>Hyrtios</i> sp. Extract in Human Colorectal Carcinoma RKO Cells with Different p53 Status

다국어 초록 (Multilingual Abstract)

<P>Drug development using marine bioresources is limited even though the ocean occupies about 70% of the earth and contains a large number of biological materials. From the screening test of the marine sponge extracts, we found <I>Hyrtios</I> sp. sponge collected from Chuuk island, Micronesia. In this study, the <I>Hyrtios</I> sp. extract was examined for anticancer activity against human colorectal carcinoma RKO cells that are wildtype for p53 and RKO-E6 that are p53 defective. The <I>Hyrtios</I> sp. extract dose-dependently inhibited viability in both cell lines. Multinucleation as an indication of mitotic catastrophe was also observed. Cytotoxicity tests gave significantly different results for RKO and RKO-E6 cells after 48 h exposure to <I>Hyrtios</I> sp. extract. In RKO cells treated with <I>Hyrtios</I> sp. extract, cell death occurred by induction of p53 and p21 proteins. In p53-defective RKO-E6 cells, <I>Hyrtios</I> sp. extract decreased expression of JNK protein and increased p21 protein. These results indicate that <I>Hyrtios</I> sp. extract induced apoptosis <I>via</I> different pathways depending on p53 status and could be a good natural product for developing new anticancer drugs. </P>