Detection of JC virus in the brains of Korean glioblastoma multiforme patients

다국어 초록 (Multilingual Abstract)

Objective : The ubiquitous human polyomavirus, JC virus(JCV) is the etiologic agent of the fatal demyelinating
central nervous system(CNS) disease, progressive multifocal leukoencephalopathy(PML). Recent studies have
reported the detection of the JCV in samples derived from several type of human neural tumors and suggested the
possible association of JCV with CNS tumors. Here we report for the first time, the presence of JCV in Korean
glioblastoma multiforme(GM) patients.
Methods : Two Korean GM patients were assayed for JCV. To detect JCV, we performed immunohistochemical
analysis using anti-JCV and anti-glial fibrillary acidic protein(GFAP) serum and polymerase chain reaction(PCR) using
primers.
Results : JCV antigen was detected in cytoplasm abundantly in cells of this tumor case. Also, GFAP
immunoreactivity was predominantly observed in cytoplasm of the cells that were morphologically bizarred
appearing astrocytes in GM. In addition, both of the large T antigen gene and the VP1 gene were detected and this
result correspond with previous result of immunohistochemistry.
Conclusion : Although it is not certain that GM is associated with the JCV, we are attempted to elucidate the
possible implication of JCV in the tumorigenesis of certain human malignant gliomas.

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