Change in Serum Concentration of Magnesium and Calcium lons Following Moderate Diffuse Axonal Injury in Rats-Preliminary Study-

다국어 초록 (Multilingual Abstract)

Objective : Traumatic brain injury has been shown to result in a decrease of the brain-free magnesium
concentration that is associated with the development of neurologic deficits. However, changes in free magnesium
homeostasis have not been characterized in other fluid compartments. We examine the ionized serum
magnesium(Mg2+) and ionized serum calcium(Ca2+) in the moderate diffuse axonal injury of rat model.
Methods : We designed a study to determine whether ionized serum magnesium(Mg2+) and / or ionized serum
calcium(Ca2+) levels correlate with moderate diffuse axonal injury(mDAI). A new ion-selective electrode was used
to determine Mg2+ and Ca2+ level in the serum, prior to and following weight-drop induced mDAI.
Results : Serum Mg2+ remained significantly depressed to about 76% of preinjury values for 3 hours (0.73±0.01
mg/dL, 0.79±0.03 mg/dL, 0.84±0.03 mg/dL at 1, 2, 3 hours after trauma, respectively) compared to control
group (1.07±0.03 mg/dL, p<0.05), but not in total serum magnesium(tMg). Mg2+/ tMg was shown a significant
decrease for first 3 hours (49%, 53.4%, 56.4% at 1, 2, 3 hours after trauma respectively) compared to control group
(70.9%, p<0.05). Head trauma resulted in small decrease of Ca2+, but there was a significant increase in the
amount of Ca2+/ Mg2+(mean value in control group : in injured group for 3 hours after trauma = 4.65±0.012 :
5.71±0.015, p<0.05). Apoptotic change was shown at 3 hours after mDAI and apoptotic index(AI) was
significantly increased at 12 and 24 hours after trauma (54.8±1.7, 51.5±3.2 at 12, 24 hours, p<0.05).
Conclusion : These findings suggest that the early decline in serum Mg2+ and increase in the amount of Ca2+/
Mg2+ following brain trauma may be a critical factor in the development of irreversible tissue injury and early
treatment with magnesium salt may be effective in histological changes following experimental traumatic brain
injury in the rat.

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