토양으로부터 단리된 Lactobacillus murinus HP-01 유래 cellulase 특성 규명 = Characterization of Cellulase from Lactobacillus murinus HP-01 Isolated from Soil

다국어 초록 (Multilingual Abstract)

The microorganisms producing cellulase were isolated from environmental soil of Duryu Park in Daegu, Korea. One strain producing cellulase was selected by forming an active zone on a trypan blue LB agar plate containing 0.5% carboxymethyl cellulose (CMC) and 0.02% trypan blue. In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99.43% of identity with Lactobacillus murinus so that the isolated strain was named Lactobacillus murinus HP-01. And then, isolated L. murinus HP-01 was cultivated on TSB-G medium including 0.5% CMC as a substrate. As a result, the strain showed the highest activity of cellulase after 2 days. In the effect of pH and temperature, cellulase from L. murinus HP-01 showed the highest activity at pH 5.0 and 50°C, respectively. On the optimal condition, the cellulase was found to be stable at pH ranging from 4.0 to 9.0, and it exhibited thermal stability with a half-life of 1.24 hours at 50 °C. In the investigation of substrate specificity for cellulosic substrates, the specific activity of the cellulase from L. murinus HP-01 was highest for CMC, followed by sigmacell cellulose, alpha cellulose, swollen avicel cellulose.