Short Communications : Characterization of Hydroxycinnamoyl-coenzyme A Shikimate Hydroxycinnamoyltransferase from Populus euramericana = Short Communications : Characterization of Hydroxycinnamoyl-coenzyme A Shikimate Hydroxycinnamoyltransferase from Populus euramericana

다국어 초록 (Multilingual Abstract)

Hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase (HCT), PeHCT was cloned from Populus euramericana. The predicted amino acid sequence revealed that PeHCT contained a conserved HXXXDG motif that was found in acyltransferase. The in vitro substrate was determined using recombinant PeHCT. The recombinant PeHCT showed substrate-specificity towards shikimic acid. In addition, PeHCT displayed higher expression in the stem than in the leaf, and its expression increased during the growth stage of the leaf. Results indicate PeHCT could be involved in lignin biosynthesis by formation of p-coumaroyl shikimate.

참고문헌 (Reference)

1 Schoch GA, "The meta-hydroxylation step in the phenylpropanoid pathway:A new level of complexity in the pathway and its regulation" 4 : 127-136, 2006

2 Franke R, "The Arabidopsis REF8 gene encodes the 3-hydroxylase of phenylpropanoid metabolism" 30 : 33-45, 2002

3 Dixon RA, "Stress-induced phenylpropanoid metabolism" 7 : 1085-1097, 1995

4 Hoffmann L, "Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis" 16 : 1446-1465, 2004

5 Hoffmann L, "Purification,cloning,and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism" 278 : 95-103, 2003

6 Sonnante G, "Novel hydroxycinnamoyl-coenzyme A quinate transferase genes from artichoke are involved in the synthesis of chlorogenic acid" 153 : 1224-1238, 2010

7 Wagner A, "Exploring lignification in conifers by silencing hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase in Pinuc radiata" 104 : 11856-11861, 2007

8 St Pierre B, "Evolution of acyltransferase genes: Origin and diversification of the BAHD superfamily of acyltransferases involved in secondary metabolism. In Recent Advances in Phytochemistry vol. 34" Elsevier Science 285-315, 2000

9 Yoon-Jung Lee, "Enzymatic Synthesis of Phenolic CoAs Using 4-Coumarate:coenzyme A Ligase (4CL) from Rice" 대한화학회 28 (28): 365-366, 2007

10 Niggeweg R, "Engineering plants with increased levels of the antioxidant chlorogenic acid" 22 : 746-754, 2004

11 Clifford MN, "Chlorogenic acids and other cinnamtesnature,occurrence and dietary burden" 79 : 362-372, 1999

12 Franke R, "Changes in secondary metabolism and deposition of an unusual lignin in the ref8 mutant of Arabidopsis" 30 : 47-59, 2002

13 Schoch G, "CYP98A3 from Arabidopsis thaliana is a 3’-hydroxylase of phenolic esters,a missing link in the phenylpropanoid pathway" 276 : 36566-36574, 2001

14 Bong Gyu Kim, "Anthocyanin Content in Rice Is Related to Expression Levels of Anthocyanin Biosynthetic Genes" 한국식물학회 50 (50): 156-10, 2007

15 D’Auria JC, "Acyltransferases in plants:a good time to be BAHD" 9 : 331-340, 2006