내염성과 관련 있는 벼의 CBF4 유전자(OsCBF4)를 벼에서 과발현 시켜 염 스트레스 조건에서 저항성이 증진된 내염성 유전자원을 개발하였다. RT-PCR을 수행하여 분리한 OsCBF4 유전자는 1,429 bp 크기...
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https://www.riss.kr/link?id=A100252727
2014
Korean
523
KCI등재후보
학술저널
109-115(7쪽)
1
0
상세조회0
다운로드국문 초록 (Abstract)
내염성과 관련 있는 벼의 CBF4 유전자(OsCBF4)를 벼에서 과발현 시켜 염 스트레스 조건에서 저항성이 증진된 내염성 유전자원을 개발하였다. RT-PCR을 수행하여 분리한 OsCBF4 유전자는 1,429 bp 크기...
내염성과 관련 있는 벼의 CBF4 유전자(OsCBF4)를 벼에서 과발현 시켜 염 스트레스 조건에서 저항성이 증진된 내염성 유전자원을 개발하였다. RT-PCR을 수행하여 분리한 OsCBF4 유전자는 1,429 bp 크기의 뉴클레오티드로 274개의 아미노산으로 구성되었고, 벼의 다른 CBF 유전자와 33~49% 상동성을 나타내었다. 형질전환 식물체는 PCR과 Southern분석으로 OsCBF4 유전자의 벼 게놈 내 도입을 확인하였다. 전이 유전자는 벼 게놈 내에 1~4사본이 도입되었고, 선발된 형질전환 계통 모두에서 전이 유전자가 강하게 발현되었다. 염 스트레스 조건에서 형질전환 식물체는 비 형질전환 벼 보다 생육 정도가 양호하였으며, 특히 CBF4-10 계통은 염 처리 후 많은 식물체가 살아남았다. Real-time PCR 분석 결과 CBF4-10 계통은 120 mM NaCl 처리 시 전이 유전자 OsCBF4 전사체 발현이 잎에서 무처리 대비 약 3배 이상 증가하였다. 결론적으로 일반 벼에 도입된 OsCBF4 전이 유전자는 내염성 증진 기능이 있으며, OsCBF4 전이 유전자의 발현이 높은 형질전환 벼 계통은 내염성 벼 육종 소재로 이용할 수 있을 것으로 평가된다.
다국어 초록 (Multilingual Abstract)
This study was conducted to isolate a salt tolerant gene and to develop salt tolerant rice for reclaimed-saline areas through genetic transformation. A rice c/DRE binding factor 4 (OsCBF4) cDNA was isolated from rice (cv. Nipponbare) using RT-PCR. The...
This study was conducted to isolate a salt tolerant gene and to develop salt tolerant rice for reclaimed-saline areas through genetic transformation. A rice c/DRE binding factor 4 (OsCBF4) cDNA was isolated from rice (cv. Nipponbare) using RT-PCR. The full-length cDNA of the CBF4 gene consists of 1,429 nucleotides and 274 amino acid residues. The OsCBF4 shares from 33 to 49% identity of deduced amino acid sequence with other CBFs of rice. In order to develop salt tolerant rice, transgenic rice plants containing the OsCBF4 gene were obtained via Agrobacterium-mediated transformation. The stable incorporation of the OsCBF4 gene into rice genome was confirmed by PCR and Southern analysis. The stable expression of introduced gene was also validated by RT-PCR analysis in T₂ plants. Biological assay of T₃ progeny of the transgenic plants in Yoshida solution containing 120 mM Nacl for 2 weeks, confirmed that the OsCBF4 confers salt tolerance to transgenic rice plants. OsCBF4transgene in the transgenic line CBF4-10 was markedly expressed up to over three-fold in the leaf by 120 mM NaCl treatment. Real-time PCR analysis revealed that the levels of the transgene expression were markedly increased under salt treatment. The transgenic line CBF4-10 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.
참고문헌 (Reference)
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1 김효진, "콩의 Pathogenesis-Related 10 유전자를 이용한 내염성 벼 형질전환 계통 개발" 한국육종학회 42 (42): 540-546, 2010
2 Allen RD, "Use of transgenic plants to study antioxidant defenses" 23 : 473-479, 1997
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5 Hsieh TH, "Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress" 130 : 618-626, 2002
6 Maghuly F, "Stress regulated expression of the Gus-marker gene (uidA) under the control of plant calmodulin and viral 35S promoters in a model fruit tree rootstock: Prunus incisa×serrula" 135 : 105-116, 2008
7 Ashraf M, "Relative memberane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.)" 63 : 266-273, 2008
8 Roxas VP, "Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress" 15 : 988-991, 1997
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10 Nakashima K, "Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration-and high-salinity-responsive gene expression" 42 : 657-665, 2000
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벼의 CAPS 마커 개발 및 줄기굵기 특성의 양적형질 유전자좌(QTL) 분석
대규모 GMO 포장에서 가뭄저항성벼(Agb0103)가 곤충다양성에 미치는 영향
배추(Brassica rapa)에서 분리한 스트레스 관련 후보 유전자 BrTSR53의 특성 분석
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2025 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2022-01-01 | 평가 | 등재학술지 선정 (계속평가) | ![]() |
2021-12-01 | 평가 | 등재후보로 하락 (재인증) | ![]() |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2015-01-01 | 평가 | 등재학술지 선정 (계속평가) | ![]() |
2014-01-01 | 평가 | 등재후보학술지 유지 (계속평가) | ![]() |
2013-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | ![]() |
2011-01-01 | 평가 | 등재 1차 FAIL (등재유지) | ![]() |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2008-04-07 | 학술지명변경 | 외국어명 : KOREAN JOURNAL OF BREEDING -> KOREAN JOURNAL OF BREEDING SCIENCE | ![]() |
2007-01-01 | 평가 | 등재 1차 FAIL (등재유지) | ![]() |
2005-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2002-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | ![]() |
1999-07-01 | 평가 | 등재후보학술지 선정 (신규평가) | ![]() |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.6 | 0.6 | 0.49 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.45 | 0.41 | 0.952 | 0.07 |