질식성 심정지 모델에서 N^6-L-phenylisopropyl Adenosine의 영향 = Effects of N6-L-phenylisopropyl Adenosine in Rats after Asphyxial Cardiac Arrest

다국어 초록 (Multilingual Abstract)

Purpose: Cardiac arrest and resuscitation produce global cerebral ischemia and reperfusion injury to the brain, which lead to high mortality and delayed neuronal death. Adenosine has been suggested as an endogenous neuroprotective molecule, acting through multiple potential mechanisms. We investigated the possible neuroprotective effects of adenosine on cerebral recovery following global ischemia induced by asphyxial cardiac arrest.
Methods: Twenty-four rats were randomized into three groups. Group Ⅰ,Ⅱ and Ⅲ had anesthesia, procedures, and asphyxia for 7 minutes and then survived to 72 hours. Group Ⅰ(n=8) was not administered N^6-L-phenylisopropyl adenosine(L-PIA). Group Ⅱ(n=8) was administered L-PIA(0.8 mg/kg), and group Ⅲ(n=8) was administered L-PIA (1.5 mg/kg), after spontaneous circulation. The dose-dependent neuroprotective effects of L-PIA were compared to the control by using a histopathological method.
Results: Histological observations of CA1 showed a more significant reduction of neuronal cell loss in groups Ⅱ and Ⅲ than in group I(p<0.05).
Histological observations of CA2 and CA3 didn't show a significant reduction of neuronal cell loss in groups Ⅱ and Ⅲ compared to group Ⅰ.
Conclusion: These results show that post-ischemic administration of adenosine protected against delayed neuronal damage in the hippocampal CA1 area following a 7-min asphyxial cardiac arrest in rats.

목차 (Table of Contents)

• 서론
• 대상과 방법
• 1. 실험동물의 분류
• 2. 수술적 조작
• 3. 심정지와 심폐소생술
• 4. 평가
• (1) 헤마톡실린-에오신(Hematoxylin-Eosin) 염색
• (2) 조직손상 정도의 평가
• 5. 통계
• 결과
• 1. 시간 변수
• 2. 혈역학적인 지표 평가
• 3. 조직손상점수
• 고찰
• 결론