Metal halide-based ligand exchange of Cd-free quantum dots for cell imaging

목차 (Table of Contents)

• I. Introduction
• 1.1 Introduction to Quantum Dots and Basic Properties -----------------------------------------------8
• 1.2 Surface Modification of QDs ------------------------------------------------------------------------10
• 1.3 Synthesis of water-soluble InP QDs and current issues ------------------------------------------12
• 1.4 Cell imaging with InP QDs --------------------------------------------------------------------------15
• 1.5 HaloTag technology ----------------------------------------------------------------------------------17
• II. Developing Zwitterionic Carboxylated ligand coated QDs for HaloTag/HaloTag ligand-mediated EGFR receptor Bioprobe
• 2.2 Experimental Section ---------------------------------------------------------------------------------18
• 2.2.1 Materials-------------------------------------------------------------------------------------------18
• 2.2.2 Characterization ----------------------------------------------------------------------------------18
• 2.2.3 RAFT polymerization of PZL, PZCL Ligands -----------------------------------------------18
• 2.2.4 Two-step ligand exchange of QDs -------------------------------------------------------------18
• 2.2.5 Dispersion stability of QDs----------------------------------------------------------------------19
• 2.2.6 Nonspecific binding test with BSA-------------------------------------------------------------19
• 2.2.7 Gel-electrophoresis measurement--------------------------------------------------------------19
• 2.2.8 FPLC measurement ------------------------------------------------------------------------------19
• 2.2.9 Synthesis of HaloTag ligand --------------------------------------------------------------------19
• 2.2.10 Synthesis and purification of QDs-HTL------------------------------------------------------20
• 2.2.11 Synthesis of QDs-HTL-HT-EGFR-Afb------------------------------------------------------20
• 2.2.12 Cell culture and Fluorescence cell imaging--------------------------------------------------20
• 2.3 Result and Discussion---------------------------------------------------------------------------------22
• 2.4. Conclusion---------------------------------------------------------------------------------------------31
• References ----------------------------------------------------------------------------------------------------32
• Acknowledgement------------------------------------------------------------------------------------------------37