운동보조제 투여가 혈중 유리지방산과 중추 TPH 발현에 미치는 영향

국문 초록 (Abstract)

본 연구는 운동보조제 투여가 지구성 운동수행력과 에너지기질 및 중추신경계 tryptophan hydroxylase (TPH) 발현에 미치는 영향을 규명하고자 Sprague-Dawley계열의 수컷 흰쥐를 무작위 표본추출법을 사용하여 운동보조제를 투여하지 않은 대조군, 운동군, 운동-글루코스투여군, 운동-카페인투여군, 그리고 운동-카르니틴투여군으로 분류하였다. 운동보조제의 투여는 체중 1㎏당 글루코스 1.4 g, 카페인 6 ㎎, 그리고 카르니틴 100 ㎎을 1 ㎖의 saline에 녹여서 3일 동안 1일 1회 운동 1시간 전 복강에 주사하였으며, NaC은 1 ㎖의 0.9 % NaCl을 동일한 방법으로 주사하였다. 운동부하는 경사도 0%에서 10 m/min 속도로 10분, 13 m/mln 속도로 10불 그리고 16 m/min의 속도로 10분간 달리도록 구성하여 1일 30분, 연속 6일 동안 실시하였다. 실험 7일 째, 운동 1시간 전에 운동보조제와 NaCl을 주사한 후 트레드일 경사도 0 %, 10 m/min 속도로 운동을 시작하여 운동부하를 점증적으로 증가시켰으며, 35 m/min 속도에서 탈진까지 운동을 실시하여 지구성 운동능력을 측정하였다. 지구성 운동수행능력은 집단간에 유의란 차이를 보였으며, 운동-카페인과 운동-카르니틴투여군의 운동수행능력이 운동-글루코스와 운동-NaCl투여군보다 유의하게 높게 나타났다. 혈중 유리지방산 농도는 운동-카페인투여군이 운동-글루코스와 운동군보다, 그리고 운동-카르니틴투여군이 운동군보다 각각 유의하게 높게 나타났다. Dorsal raphe 부위의 TPH 발현은 집단간 유의한 차이를 보였으며, 비운동군과 복교하기 모든 운동-투여군에서 탈진운동시 TPH 발현이 유의하게 증가한 것으로 나타났으나, 운동-카페민투여군이 운동군보다 유의하게 감소하였다. 본 연구결과 운동보조제 중에서 카피이과 카르니틴 투여는 혈중 유리지방산 농도를 증가시키고, 카페인은 중추피로 전구물질인 TPH 발현을 감소시킴으로서 지구성 운동수행능력을 향상시킬 수 있을 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

The pupose of this study was to investigate the effects of infusion of ergogenic acids on blood tree fat acid (FFA) and tryptophan hydroxylase (TPH) expression in dorsal raphe. Male Sprague-Dawley rats were randomly divided into control (CON), exercise-with-NaCl (EXE), exercise-with-glucose (GLU), exercise-with-caffeine (CAF), exercise-with-carnitine (CAR) groups. Rats were injected intraperitoneally the glucose (1.4 g/㎏), caffeine (6 ㎎/㎏), carnitine (100 ㎎/㎏), and 0.9 % NaCl h prior to the exercise for 3 consecutive days . Animals of the exercise groups were put on running for 30 min once a day for 6 consecutive days. On the 7th day of the experiment, rats were run on a treadmill at a speed of 35 m/min until exhaustion. In the present results, exercise time to exhaustion in the CAF and CAR groups was significantly increased compared to the EXEI and GLU groups. Blood FFA levels in the CAF group was significantly increased compared to the EXE and GLU groups, and the CAR group was than higher the EXE group. TPH expression of the dorsal raphe in the EXE, GLU, CAF, CAR groups was significantly increased compared to the CON group. However, CAF group deceased TPH expression significantly compared to the group. These results suggest that caffeine, carnitine administration could promote fat oxidation, resulting in higher endurance performance during exercise, and especially the caffeine, inhibiting TPH expression, may play an ergogenic role in exercise performance by reducing fatigue.

목차 (Table of Contents)

• Abstract
• 국문초록
• Ⅰ. 서론
• Ⅱ. 연구방법
• Ⅲ. 연구결과
• Ⅳ. 논의
• Ⅴ. 결론
• 참고문헌

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