Analysis of the Hot-spot Mutant Protein from Cancer Patients based Localized Surface Plasmon Resonance of Single Gold Nanoparticle = Analysis of the Hot-spot Mutant Protein from Cancer Patients based Localized Surface Plasmon Resonance of Single Gold Nanoparticle

다국어 초록 (Multilingual Abstract)

The p53 protein is a well-known tumor suppressor that plays an important role in preventing cancer. The mutations of p53 such as functional damage of its DNA binding domain lead to tumors. Specially, the mutants of p53 at hot spot have unique changes of local electron density that affect specificity of DNA binding compared with the wild types. This can be detected by Rayleigh light scattering according to the changes of localized surface plasmon resonance (LSPR). The LSPR Δλmax shift of mutant p53 proteins is sifnificantly lower than that of wild types. These differences propose that hot-spot mutants can be easily detected by local electron density alterations that disturbs the specificity of DNA binding domain of p53 on the nanoplasmonic sensor. By this strategy, the nanoplasmonic sensor can detect mutant p53 proteins from samples of breast cancer patients and thus suggesting itself as a promising tool for using in cancer diagnosis and predict the effect from adjuvant treatment.