Changes of Ginsenoside Content by Mushroom Mycelial Fermentation in Red Ginseng Extract

다국어 초록 (Multilingual Abstract)

To obtain microorganisms for the microbial conversion of ginsenosides in red ginseng extract (RGE), mushroom mycelia were used for the fermentation of RGE. After fermentation, total sugar contents and polyohenol contents of the RGEs fermented with various mushrooms were not a significant increase between RGE and the ferments. But uronic acid content was relatively higher in the fermented RGEs cultured with Lentus edodes (2155.6 ㎍/mL), Phelllinus linteus (1690.9 ㎍/mL) and Inonotus obliquus 26137 and 26147 (1549.5 and 1670.7 ㎍/mL) compared to the RGE (1307.1 ㎍/mL). The RGEs fermented by Ph. linteus, Cordyceps militaris, and Grifola frondosa showed particularly high levels of total ginsenosides (20018.1, 17501.6, and 16267.0 ㎍/mL, respectively). The ferments with C. militaris (6974.2 ㎍/mL), Ph. linteus (9109.2 ㎍/mL), and G. frondosa (7023.0 ㎍/mL) also showed high levels of metabolites (sum of compound K, Rh₁, Rg?, Rk₁, Rg₃, and Rg₂) compared to RGE (3615.9 ㎍/mL). Among four different RGE concentrations examined, a 20 brix concentration of RGE was favorable for the fermentation of Ph. linteus. Maximum biotransformation of ginsneoside metabolites (9395.5 ㎍/mL) was obtained after 5 days fermentation with Ph. linteus. Maximum mycelial growth of 2.6 ㎎/mL was achieved at 9 days, in which growth was not significantly different during 5 to 9 days fermentation. During fermentation of RGE by Ph. linteus in a 7 L fermenter, Rg₃, Rg?, and Rk₁ contents showed maximum concentrations after 5 days similar to flask fermentation. These results confirm that fermentation with Ph. linteus is very useful for preparing minor ginsenoside metabolites while being safe for foods.

목차 (Table of Contents)

• INTRODUCTION
• MATERIALS AND METHODS
• RESULTS
• DISCUSSION
• ACKNOWLEDGEMENTS
• REFERENCES

참고문헌 (Reference)

1 박창규, "균사체를 이용한 수삼 고체발효물의 화학적 조성 및 면역 활성" 한국식품영양과학회 38 (38): 1145-1152, 2009

2 LEE, BO HYUN, "Transformation of the Glycosides from Food Materials by Probiotics and Food Microorganisms" 한국미생물·생명공학회 16 (16): 497-504, 2006

3 Han Y, "Transformation of bioactive compounds by Fusarium sacchari fungus isolated from the soil-cultivated ginseng" 55 : 9373-9379, 2007

4 Wakabayashi C, "The expression of in vivo anti-metastatic effect of ginseng protopanaxatriol saponins is mediated by their intestinal bacterial metabolites after oral administration" 14 : 180-185, 1997

5 Lou DW, "Solid-phase extraction and high-performance liquid chromatography for simultaneous determination of important bioactive ginsenosides in pharmaceutical preparations" 62 : 349-354, 2005

6 Hasegawa H, "Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins" 63 : 436-440, 1997

7 Kobashi K, "Relation of intestinal bacteria to pharmacological effects of glycosides" 16 : 1-7, 1997

8 Hasegawa H, "Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid" 95 : 153-157, 2004

9 Hasegawa H, "Pharmacokinetics of ginsenoside deglycosylated by intestinal bacteria and its transformation to biologically active fatty acid esters" 23 : 298-304, 2000

10 Kim SJ, "Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng (Panax ginseng CA Meyer)" ELSEVIER SCIENCE BV 56 (56): 401-406, 2007

11 Chang KH, "Optimization of the enzymatic production of 20(S)-ginsenoside Rg(3) from white ginseng extract using response surface methodology" 26 : 181-186, 2009

12 Hasegawa H, "Oleoyl triterpene glycoside biosynthesized from ginseng suppresses growth and metastasis of murine melanoma B16-F10 tumor via immunostimulation" 17 : 186-193, 2000

13 Blumenkrantz N, "New method for quantitative determination of uronic acids" 54 : 484-489, 1973

14 Bae EA, "Metabolism of ginsenoside R(c) by human intestinal bacteria and its related antiallergic activity" 25 : 743-747, 2002

15 Kobashi K, "Metabolism of drugs by intestinal bacteria" 11 : 9-23, 1992

16 Bae EA, "Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities" 25 : 58-63, 2002

17 He W, "Inhibitory effects of ginsenosides from the root of Panax ginseng on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation, and translocation of cytosolic compounds to plasma membrane in human neutrophils" 56 : 1921-1927, 2008

18 Wakabayashi C, "In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration" 9 : 411-417, 1997

19 Tachikawa E, "In vitro inhibition of adrenal catecholamine secretion by steroidal metabolites of ginseng saponins" 66 : 2213-2221, 2003

20 Park EK, "Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities" 133 : 113-120, 2004

21 Lu JM, "Ginseng compounds: an update on their molecular mechanisms and medical applications" 7 : 293-302, 2009

22 Dai-SikLee, "Fecal Metabolic Activities of Herbal Components to Bioactive Compounds" 대한약학회 25 (25): 165-169, 2002

23 Su JH, "Enzymatic transformation of ginsenoside Rg3 to Rh2 using newly isolated Fusarium proliferatum ECU2042" 38 : 113-118, 2006

24 Ko SR, "Enzymatic preparation of ginsenosides Rg2, Rh1, and F1 from protopanaxatriol-type ginseng saponin mixture" 69 : 285-286, 2003

25 Hyun-Jeong Kim, "Effects of Fermented Red Ginseng Extracts on Hyperglycemia in Streptozotocin-induced Diabetic Rats" 고려인삼학회 34 (34): 104-112, 2010

26 Kim M, "Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age" 11 : 10-16, 1987

27 Tawab MA, "Degradation of ginsenosides in humans after oral administration" 31 : 1065-1071, 2003

28 Tawab MA, "Degradation of ginsenosides in humans after oral administration" 31 : 1065-1071, 2003

29 Han BH, "Degradation of ginseng saponins under mild acidic conditions" 44 : 146-149, 1982

30 Dubois M, "Colorimetric method for determination of sugars and related substances" 28 : 350-356, 1956

31 Shibata S, "Chemical studies on the oriental plant drugs. XVII. The prosapogenin of the ginseng saponins (ginsenosides-Rb1, -Rb2, and -Rc)" 14 : 1157-1161, 1966

32 Kim BH, "Biotransformation of Korean Panax ginseng by Pectinex" 29 : 2472-2478, 2006

33 Lee SJ, "Antitumor activity of a novel ginseng saponin metabolite in human pulmonary adenocarcinoma cells resistant to cisplatin" 144 : 39-43, 1999

34 Dey L, "Anti-diabetic and anti-obese effects of ginseng berry extract: comparison between intraperitoneal and oral administrations" 30 : 645-647, 2002

35 Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent" 299 : 152-178, 1999