Proteomic Analysis of Parasitization by Cotesia plutellae against Diamondback Moth, Plutella xylostella

다국어 초록 (Multilingual Abstract)

Parasitization by an endoparasitoid wasp, Cotesia plutellae, against diamondback moth (DBM), Plutella xylostella, is fostered by maternal and embryonic factors such as polydnavirus, venom, and teratocyte. Genome of C. plutellae bracovirus ( CpBV') possessed at least 19 DNA segments, which ranged from 6.8 to over 30 Kb in size and were not equimolar in abundance. Teratocytes began to release into hemolymph three days after parasitization. Their cell size increased more than two fold, but cell density was not changed until six days after parasitization. After six days, teratocytes began to disappear. With these parasitizing factors, proteomic analysis was conducted in non-parasitized and parasitized DBM every 24h after parasitization to find out parasitization-specific genes. About 2,000 protein spots were resolved in two-dimensional gel. A clustering expression analysis indicated that 21 proteins of DBM were highly modulated in their expression level by the parasitization. Among such responsive proteins, 16 spots were specifically expressed in non-parasitized DBM, while five spots were only in parasitized DBM. The internal N-terminal amino acid sequences of five parasitization- specific proteins were identified by MALDI-TOF analysis and compared with those of the known proteins available in public databases. Sequence analysis revealed that the five parasitization-specific proteins shared slight homologies with several transcriptional factors or protein kinases. These five proteins were different in their expression profiles after parasitization. These proteomic analyses also reflect that parasitization by C. plutellae modulates various normal gene expressions of DBM probably by parasitization- specific factors.

참고문헌 (Reference)

1 "in Virus taxonomy, Eds. M.H.V., van Regenmortel, C.M. Fauquet, D.H.L. Bishop, E.B" Academic Press 253-260, 2000

2 "in Parasites and pathogens of insects" Academic Press 145-165, 1993

3 "and management of the diamondback moth" 275-301, 1993

4 "Virus with a multipartite superhelical DNA genome from the inchneumonid parasitoid Campoletis sonorensis" 859-870, 1982

5 "The giant cells produced by Telenomus remus" 89-94, 1973

6 "The biology of Platygaster herrickii a parasite of the Hessian fly" 1937199-212

7 "Similar polydnavirus genes of two parasitoids" 463-471, 2003

8 "Protection by immune disguise a new lesson from a parasitoid wasp" CSIRO Publishing 23-37, 2000

9 "Polydnavirus replication and ovipositional habit of Cotesia plutellae" 43 : 225-231, 2004

10 "Polydnavirus infection inhibits translation of specific growth-associated host proteins" 263-270, 1997

11 "Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels" 850-858, 1996

12 "Inhibitory effect of Cotesia plutellae bracovirus on development of a non-natural host" 43 : 217-223, 2004

13 "In vitro culture of the teratocytes of Trissolcus basalis (Hymenoptera, Scelionidae) and their rements for host- derived components" 176-184, 2001

14 "Host physiological changes due to parasitism of a braconid wasp : Cotesia plutellae, on diamondback moth, Plutella xylostella." 39-44, 2004

15 "Host haemocyte inactivation by an insect parastoid: transient expression of a polydnavirus gene" 777 : 2653-2662, 1996

16 "Growth patterns of teratocytes in the immature stages of Trissolcus basalis an egg parasitoid of Nezara viridula" off hyme (off hyme): 323-336, 1992

17 "Free serosal cells originating from embryo of the wasp Diadromus pulchellus in the pupal fatbody of parasitized leek-moth" 479-484, 1999

18 "Evaluation of teratocytes functions" 159-166, 1990

19 "Developmental disruption of Pseudoplusia includens and Heliothis virescens larvae by the calyx fluid and venom Microplitis demolitor" 131-145, 1991

20 "Development and morphology of teratocytes in Encarsia berlesei and Encarsia citrina: first record for Chalcidoidea" 49 : 1063-1071, 2003

21 "Cultivation of teratocyes of the egg parasitoid Telenomus heliothidis" hyme (hyme): 361-367, 1985

22 "Cotesia kariyai teratocytes: growth and development" 31-41, 2001

23 "Correlating the initiation of virus replication with a specific pupal developmental phase of an ichneumonid parasitoid" 387-398, 1983

24 "Classification and biology of braconid wasps Handbooks for the identification of British insects" hyme (hyme): 1-126, 1991

25 "Activity of juvenile hormone esterase of diamondback moth is not inhibited by parasitiosm of Cotesia plutellae" 283-287, 2004

26 "A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels" 361-363, 1980