특정 세균으로 유도된 백서의 치수염에서 T 세포 아군집의 분포와 interleukin-2, interferon-γ, interleukin-4의 수준에 관한 연구 = THE LEVELS OF INTERLEUKIN-2, INTERFERON-γ, INTERLEUKIN-4 AND TLYMPHOCYTE SUBPOPULATIONS IN RAT PULPAL INFLAMMATION INDUCED EXPERIMENTALLY BY SPECIFIC BACTERIA

다국어 초록 (Multilingual Abstract)

Immune response associated with bacterial infection involve inflammatory cells. Clinical symptoms and pathologic features are particularly influenced by the predominant cells. Among inflammatory cells. T cells have the heterogenity. T cells may develop into the mature cell expressing the cell surface markers with different functions and T helper cells are categorized into Th1 and Th2 cells based on their different patterns of cytokine production. The objective of this study was to investigate the change of expression of surface markers on T cells and the Th1/Th2 immune response in pulpal inflammation associated with specific bacteria. We experimentally induced pulpal inflammation in rat incisors by drilling without coolant and innoculated with Streptococcus mutans (S.M. group), Porphyromonas endodontalis (P.E.group), or only sterile cotton (control group). After 1, 2, and 5 days, mandibular incisors were extracted and the pulptissues were extirpated. The expressions of IL-2 recepters (CD25) and ICAM-1 (CD-54) on CD4+ and CD8+ cells in the pulps were determined using a flow cytometer, and the concentration of IL-2, IFN-γ, and IL-4 was measured by enzyme-linked immunosorbent assay.
The results were as follows;
1. In the S.M.group. CD4+ cells were more increased at 2nd day than 1st day and in the P.E.group, CD8+ cells were more increased at 2nd day than 1st day.
2. The percentages of CD4+, CD4+25+ and CD4_54+ cells were decreased in the pulp tissues at 5th day after irritation in all groups.
3. The ratios of CD4+/CD8+, CD4+/CD4+25+ and CD4+/CD4+54+ in the pulps at 2nd day after irritation by P.endodontalis were significantly lower than the other groups.
4. The higher concentrations of IFN-γ than IL-4 in the pulps at 2nd day after irritation by P.endodontalis showed that T helper 1 reaction were predominant in the early stage of the pulpal in flammation induced by P.endodontalis.
5. The higher concentrations of IL-4 than IFN-γ in the pulps at 1st day and 5th day after irritation by S.mutans were measured but the differences were not significant.