땅빈대 추출물의 세포 보호 효과 및 성분 분석에 관한 연구 = Cellular Protective Effect and Component Analysis of Euphorbia humifusa Extracts

다국어 초록 (Multilingual Abstract)

In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction (3.68 µg/mL) and aglycone fraction (3.15 µg/mL) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activity (OSC50) of Euphorbia humifusa extract on ROS generated in Fe 3+-EDTA/H2O2 system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.43 µg/mL) and aglycone fraction (0.35 µg/mL) of extract showed higher ROS scavenging activity than L-ascorbic acid (1.50 µg/mL). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner (5~25 µg/mL), and was more effective than (+)-α-tocopherol, lipid peroxidation chain blocker.
Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging 1O2 and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.

참고문헌 (Reference)

1 배기환, "한국의 약용식물" 교학사 277-, 2000

2 허성일, "땅빈대 추출물의 항산화 활성 및 세포독성 효과" 한국생약학회 39 (39): 295-299, 2008

3 Packer,L, "Ultraviolet radiation (UVA, UVB) and skin antioxidants. In: Free radical damage and its control" Elsevier Science, St. Louis 239-253, 1994

4 Jurkiewicz, B. A, "Ultraviolet lightinduced free radical formation in skin:An electron paramagnetic resonance study" 59 : 1-4, 1994

5 Jurkiewicz, B. A., "Ultraviolet lightinduced free radial formation in skin:An electron paramagnetic resonance study" 59 (59): 1-4, 1994

6 Zheng, P, "UVA induced biochemical changes in hairless mouse skin:A comparison to UVB-Induces Damage" 100 : 194-199, 1992

7 McCord, J. M, "Superoxide dismutase an enzymic function for erythrocuprein" 244 : 6049-6103, 1969

8 Fantone, J. C, "Role of oxygenderived free radicals and metabolites in leukocyte dependent inflammatory reaction" 107 (107): 395-418, 1982

9 Davies,K.J.A, "Protein damage and degradation by oxygen radical" 262 (262): 9902-9907, 1987

10 Thomas, J. P, "Photogenenration of singlet oxygen by membrane bound hematoporphyrin derivative" 47 : 79s-, 1988

11 Scharffetter-Kochanek,K, "Photoaging of the connective tissue of skin:Its prevention and therapy,antioxidants in disease mechanism and therapy" 38 : 639-655, 1997

12 Cheseman, K. H, "Free radicals inmedicine" 49 (49): 566-576, 1993

13 Darr, D, "Free radicals in cutameous biology" 102 : 671-675, 1994

14 Jurkeiwicz, B. A, "Effect of topically applied tocopherol on ultraviolet radiationmediated free radical demage in skin" 104 : 484-488, 1995

15 Park,S.N, "Dissertation" Seoul National Univ. 1989

16 Foote,C.S, "Definition of type I and type II photosensitized oxidation" 54 : 659-666, 1991

17 Cha, B. C, "Antioxidative effect of domestic plants" 28 (28): 15-20, 1997